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Lysosomal exoglycosidases and cathepsin D in colon adenocarcinoma.

AbstractINTRODUCTION:
Changes in the structure of membrane glycoconjugates and activity of glycosidases and proteases are important in tumor formation.
OBJECTIVES:
The aim of the study was to compare the specific activity of lysosomal exoglycosidases: N-acetyl-β-D-hexosaminidase (HEX), its isoenzymes A (HEX A) and B (HEX B), β-D-galactosidase (GAL), α-fucosidase (FUC), and α-mannosidase (MAN) with the activity of cathepsin D (CD) in serum, urine, and carcinoma tissue of patients with colon adenocarcinoma.
PATIENTS AND METHODS:
The specific activity of HEX, HEX A, HEX B, GAL, FUC, MAN, and CD was assayed in serum, urine, and carcinoma tissue of 12 patients with colon adenocarcinoma.
RESULTS:
Lysosomal exoglycosidases and CD have similar specific activity in colon adenocarcinoma tissue and urine, which is higher than their activity in serum (with the exception of the highest specific activity of CD in urine). A positive correlation was observed between the specific activity of CD and that of HEX, HEX A, FUC, and MAN in the carcinoma tissue and urine as well as between CD and GAL in the urine of patients with colon adenocarcinoma. Negative correlations were observed between protein levels and the specific activity of HEX, HEX A, FUC, MAN, and CD in the carcinoma tissue and urine, and between protein levels and GAL in urine.
CONCLUSIONS:
Increased degradation and remodeling of glycoconjugates in the colon adenocarcinoma tissue is reflected by increased specific activity of exoglycosidases and CD. The results suggest a strong effect of exoglycosidase action on tissue degradation and a potential role of exoglycosidases in the initiation of proteolysis.
AuthorsNapoleon Waszkiewicz, Beata Zalewska-Szajda, Sławomir D Szajda, Alina Kępka, Magdalena Waszkiewicz, Wiesława Roszkowska-Jakimiec, Marzena Wojewódzka-Żeleźniakowicz, Anna J Milewska, Jacek Dadan, Agata Szulc, Krzysztof Zwierz, Jerzy R Ladny
JournalPolskie Archiwum Medycyny Wewnetrznej (Pol Arch Med Wewn) Vol. 122 Issue 11 Pg. 551-6 ( 2012) Poland
PMID23111620 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Biomarkers, Tumor
  • Isoenzymes
  • beta-Galactosidase
  • alpha-Mannosidase
  • Hexosaminidase A
  • Hexosaminidase B
  • beta-N-Acetylhexosaminidases
  • Cathepsin D
Topics
  • Adenocarcinoma (enzymology, metabolism)
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor (metabolism)
  • Cathepsin D (metabolism)
  • Colonic Neoplasms (enzymology, metabolism)
  • Female
  • Hexosaminidase A (metabolism)
  • Hexosaminidase B (metabolism)
  • Humans
  • Isoenzymes (metabolism)
  • Lysosomes (metabolism)
  • Male
  • Middle Aged
  • Predictive Value of Tests
  • Sensitivity and Specificity
  • Serum (metabolism)
  • alpha-Mannosidase (metabolism)
  • beta-Galactosidase (metabolism)
  • beta-N-Acetylhexosaminidases (metabolism)

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