There is currently no validated animal model for evaluating the potential allergenicity of food
proteins. This study aimed to compare the
allergic reactions between BN and Wistar rats after oral exposure to
ovalbumin (OVA) by studying immune responses and clinical manifestations. Female BN and Wistar rats were orally exposed to OVA on days 1 and 14, and thereafter daily from day 15 to day 42. Sera and plasma were screened for OVA-specific
antibodies and
histamine. On day 49, all the OVA-sensitized animals were orally challenged with OVA before blood pressure was measured. One day later (on day 50), histopathology and differential cell counts were performed. The results indicate that oral exposure of BN rats to OVA yielded
IgE,
IgG, and
IgG(2a) antibody responses that were generally of higher levels than those observed in Wistar rats (p < 0.05). However, the Wistar rats presented with more serious clinical manifestations and histopathologic changes that could have serious implications for any OVA-induced
anaphylaxis. The studies here proved that OVA-sensitized BN and Wistar rats evinced different immune responses and clinical manifestations; these outcomes suggested that the two rat strains might differ in their immunologic mechanisms of
allergy and that there was no correlation between immune responses and the severity of clinical symptoms. To be clear, the data from these studies should be viewed as 'preliminary', as only a single
protein allergen was examined. Accordingly, further studies are needed to compare the
allergic reactions between BN and Wistar rats by using purified strong-, weak-, and non-allergenic
proteins based on the experiments reported here.