Self-assembly of conjugated polymer on hybrid nanospheres for cellular imaging applications.

Abstract

A new kind of hybrid core-shell nanosphere was fabricated by combining the in situ formation of Au nanoparticles and covalent cross-linking of biocompatible carboxymethyl starch dialdehyde (CMSD) and chitosan (CTS). When the fluorescent dye poly[9,9'-bis(6″-(N,N,N-trimethylammonium)-hexyl)fluorene-2,7-ylenevinylene-co-alt-1,4-phenylene dibromide] (PFV) was assembled on the surface of the hybrid nanospheres through electrostatic attraction, these biocompatible hybrid nanospheres exhibited metal-enhanced fluorescence effects. The fluorescence intensity of (CTS-Au)@CMSD/PFV hybrid nanosphere is 1.43 times that of CTS-CMSD/PFV hybrid nanospheres lacking Au nanoparticle. In addition, the (CTS-Au)@CMSD/PFV hybrid nanospheres exhibit excellent biodegradability upon exposure to enzymatic aqueous solution and good biocompatibility when cocultured with HeLa cervical carcinoma cells; these advantages make them attractive for cellular imaging and biological analysis and detection.

• Authors: Bihua Xia, Xiaoyu Wang, Fang He, Qianling Cui, Lidong Li
• Journal: ACS applied materials & interfaces (ACS Appl Mater Interfaces) Vol. 4 Issue 11 Pg. 6332-7 (Nov 2012) ISSN: 1944-8252 [Electronic] United States
• PMID: 23101490 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Contrast Media
• Polymers

Topics

• Contrast Media (chemical synthesis)
• HeLa Cells
• Humans
• Materials Testing
• Microscopy, Fluorescence (methods)
• Nanospheres (chemistry)
• Particle Size
• Polymers (chemistry)