Pine nut allergy: clinical features and major allergens characterization.

Abstract

SCOPE:

The aims of this study were to evaluate IgE-mediated hypersensitivity to pine nut with details of clinical reactions and to characterize major pine nut allergens.

METHODS AND RESULTS:

The study included ten consecutive teenagers and adults diagnosed with IgE-mediated clinical allergy to pine nut. Two major pine nut allergens were purified and identified and the secondary structures and susceptibility to digestion were characterized. Severe reactions represent 80% of allergic reactions to pine nut in this study. Moreover, 70% of the patients were monosensitized to this nut. Two major allergens with molecular weights of 6 and 50 kDa were purified and identified as albumin and vicilin, respectively. The 6 kDa protein (albumin), rich in α-helix content, was far more stable to peptic and tryptic digestion as compared with 50 kDa protein (vicilin), which was quickly broken down. The secondary structure of the purified 50 kDa protein showed 41% β-sheet, 5% α-helix, and 54% random coil and/or loops.

CONCLUSION:

Eighty percent of allergic reactions to pine nut in the ten patients included in this study were severe. Most patients (70%) were monosensitized to this nut. Two major allergens with molecular weights of 6 and 50 kDa were purified and identified as albumin and vicilin, respectively.

• Authors: Beatriz Cabanillas, Hsiaopo Cheng, Casey C Grimm, Barry K Hurlburt, Julia Rodríguez, Jesús F Crespo, Soheila J Maleki
• Journal: Molecular nutrition & food research (Mol Nutr Food Res) Vol. 56 Issue 12 Pg. 1884-93 (Dec 2012) ISSN: 1613-4133 [Electronic] Germany
• PMID: 23081934 (Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
• Copyright: © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Albumins
• Allergens
• Seed Storage Proteins
• Immunoglobulin E
• vicilin protein, plant
• Trypsin
• Pepsin A

Topics

• Adolescent
• Adult
• Albumins (immunology)
• Allergens (adverse effects, chemistry, immunology)
• Circular Dichroism
• Cloning, Molecular
• Cross Reactions (immunology)
• Double-Blind Method
• Electrophoresis, Polyacrylamide Gel
• Female
• Humans
• Immunoglobulin E (blood, immunology)
• Male
• Molecular Weight
• Nut Hypersensitivity (diagnosis, immunology)
• Nuts (chemistry, immunology)
• Pepsin A (metabolism)
• Seed Storage Proteins (immunology)
• Sequence Analysis, DNA
• Trypsin (metabolism)
• Young Adult