[Immunological characteristics of the recombinant major pollen allergen pTSX2 of Humulus scandens].

Abstract

OBJECTIVE:

To identify the immunological characteristics of the recombinant major pollen allergen pTSX2 of Humulus scandens and evaluate its safety in immunotherapy of allergic asthma in mice.

METHODS:

Western blotting was used to characterize the immunological properties of pTSX2, and its immunogenicity in normal mice was evaluated by detecting sIgG and sIgE levels. The mouse models of allergic asthma were immunized with pTSX2 and examined for sIgE and sIgG levels, total cells and eosinophils percentage in BALF, interleukin-4 (IL-4) and interferon-γ (IFN-γ) levels in BALF and spleen homogenate, and changes in lung pathologies.

RESULTS:

Western blotting showed that pTSX2 reacted with the majority (about 70%) of sera from patients allergic to Humulus pollen. In normal mice, pTSX2 mainly induced the production of sIgG. In mouse models of allergic asthma, intervention with pTSX2 caused a significant reduction of sIgE and an increase of sIgG (P<0.05), significantly decreased the total cells and eosinophils in BALF (P<0.05), obviously lowered IL-4 but increased IFN-γ in BALF and spleen homogenate (P<0.05), and diminished inflammatory cell infiltration and percentage of eosinophils in the lung tissues.

CONCLUSIONS:

pTSX2 shows a definite therapeutic effect and safety in the treatment of allergic asthma in mice possibly by inhibiting sIgE and inducing sIgG production, suppressing airway allergic inflammation and regulating the balance between Thl and Th2.

• Authors: Yuanyuan Wu, Xiuzhen Sun, Manxiang Li, Yun Liu, Guizuo Wang, Jiamei Lu
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 32 Issue 10 Pg. 1431-4 (Oct 2012) ISSN: 1673-4254 [Print] China
• PMID: 23076178 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Allergens
• Immunoglobulin G
• Interleukin-4
• Immunoglobulin E
• Interferon-gamma

Topics

• Allergens (immunology)
• Animals
• Asthma (immunology, therapy)
• Bronchoalveolar Lavage Fluid (immunology)
• Disease Models, Animal
• Female
• Humans
• Humulus (immunology)
• Immunoglobulin E (blood)
• Immunoglobulin G (blood)
• Immunotherapy
• Interferon-gamma (analysis)
• Interleukin-4 (analysis)
• Mice
• Mice, Inbred BALB C
• Pollen (immunology)
• Th1 Cells (cytology, immunology)
• Th2 Cells (cytology, immunology)