Effectiveness and molecular interactions of the clinically active mTORC1 inhibitor everolimus in combination with tamoxifen or letrozole in vitro and in vivo.

Abstract

INTRODUCTION:

Strategies to improve the efficacy of endocrine agents in breast cancer (BC) therapy and to delay the onset of resistance include concomitant targeting of the estrogen receptor alpha (ER) and the mammalian target of rapamycin complex 1 (mTORC1), which regulate cell-cycle progression and are supported by recent clinical results.

METHODS:

BC cell lines expressing aromatase (AROM) and modeling endocrine-sensitive (MCF7-AROM1) and human epidermal growth factor receptor 2 (HER2)-dependent de novo resistant disease (BT474-AROM3) and long-term estrogen-deprived (LTED) MCF7 cells that had acquired resistance associated with HER2 overexpression were treated in vitro and as subcutaneous xenografts with everolimus (RAD001-mTORC1 inhibitor), in combination with tamoxifen or letrozole. End points included proliferation, cell-cycle arrest, cell signaling, and effects on ER-mediated transactivation.

RESULTS:

Everolimus caused a concentration-dependent decrease in proliferation in all cell lines, which was associated with reductions in S6 phosphorylation. Everolimus plus letrozole or tamoxifen enhanced the antiproliferative effect and G1-accumulation compared with monotherapy, as well as increased phosphorylation (Ser10) and nuclear accumulation of p27 and pronounced dephosphorylation of Rb. Sensitivity was greatest to everolimus in the LTED cells but was reduced by added estrogen. Increased pAKT occurred in all circumstances with everolimus and, in the BT474 and LTED cells, was associated with increased pHER3. Decreased ER transactivation suggested that the effectiveness of everolimus might be partly related to interrupting cross-talk between growth-factor signaling and ER. In MCF7-AROM1 xenografts, letrozole plus everolimus showed a trend toward enhanced tumor regression, versus the single agents. In BT474-AROM3 xenografts, everolimus alone was equally effective at reducing tumor volume as were the combination therapies.

CONCLUSIONS:

The results provide mechanistic support for recent positive clinical data on the combination of everolimus and endocrine therapy, as well as data on potential routes of escape via enhanced HER2/3 signaling. This merits investigation for further improvements in treatment efficacy.

• Authors: Lesley-Ann Martin, Sunil Pancholi, Ian Farmer, Stephanie Guest, Ricardo Ribas, Marion T Weigel, Allan M Thornhill, Zara Ghazoui, Roger A'Hern, Dean B Evans, Heidi A Lane, Stephen R Johnston, Mitch Dowsett
• Journal: Breast cancer research : BCR (Breast Cancer Res) Vol. 14 Issue 5 Pg. R132 (Oct 17 2012) ISSN: 1465-542X [Electronic] England
• PMID: 23075476 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Hormonal
• Multiprotein Complexes
• Nitriles
• Protein Kinase Inhibitors
• Receptors, Estrogen
• Triazoles
• Tamoxifen
• Letrozole
• Everolimus
• Receptor, ErbB-3
• Mechanistic Target of Rapamycin Complex 1
• Proto-Oncogene Proteins c-akt
• TOR Serine-Threonine Kinases
• Sirolimus

Topics

• Animals
• Antineoplastic Agents, Hormonal (administration & dosage, pharmacology)
• Breast Neoplasms (drug therapy, metabolism, pathology)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Nucleolus (metabolism)
• Cell Proliferation (drug effects)
• Disease Models, Animal
• Drug Resistance, Neoplasm
• Everolimus
• Female
• Humans
• Letrozole
• Mechanistic Target of Rapamycin Complex 1
• Mice
• Multiprotein Complexes (antagonists & inhibitors, metabolism)
• Nitriles (administration & dosage, pharmacology)
• Phosphorylation
• Protein Kinase Inhibitors (administration & dosage, pharmacology)
• Protein Transport
• Proto-Oncogene Proteins c-akt (metabolism)
• Receptor, ErbB-3 (metabolism)
• Receptors, Estrogen (metabolism)
• Signal Transduction (drug effects)
• Sirolimus (administration & dosage, analogs & derivatives, pharmacology)
• TOR Serine-Threonine Kinases (antagonists & inhibitors, metabolism)
• Tamoxifen (administration & dosage, pharmacology)
• Triazoles (administration & dosage, pharmacology)
• Xenograft Model Antitumor Assays