Invasion is a critical step in lung
tumor progression. The interaction between
tumor cells and their surroundings may play an important role in
tumor invasion and
metastasis. To better understand the mechanisms of
tumor invasion and tumor-microenvironment interactions in lung
tumors, total
RNA was isolated from the inner
tumor,
tumor invasion front, adjacent lung, and distant normal lung tissue from 17 patients with primary squamous cell lung
carcinoma using punch-aided
laser capture microdissection.
Messenger RNA expression profiles were obtained by microarray analysis, and
microRNA profiles were generated from eight of these samples using TaqMan Low Density Arrays. Statistical analysis of the expression data showed extensive changes in gene expression in the inner
tumor and
tumor front compared with the normal lung and adjacent lung tissue. Only a few genes were differentially expressed between
tumor front and the inner
tumor. Several genes were validated by immunohistochemistry. Evaluation of the
microRNA data revealed
zonal expression differences in nearly a fourth of the
microRNAs analyzed. Validation of selected
microRNAs by in situ hybridization demonstrated strong expression of hsa-miR-196a in the inner
tumor; moderate expression of hsa-miR-224 in the inner
tumor and
tumor front, and strong expression of
hsa-miR-650 in the adjacent lung tissue. Pathway analysis placed the majority of genes differentially expressed between
tumor and nontumor cells in intrinsic processes associated with
inflammation and extrinsic processes related to lymphocyte physiology. Genes differentially expressed between the inner
tumor and the adjacent lung/normal lung tissue affected pathways of
arachidonic acid metabolism and
eicosanoid signaling.