Positive relationship between a polymorphism in Helicobacter pylori neutrophil-activating protein a gene and iron-deficiency anemia.

Abstract

BACKGROUND:

Numerous studies have suggested a link between iron-deficiency anemia (IDA) and Helicobacter pylori infection. Previously, we found that strains isolated from IDA patients showed higher levels of Fe ion uptake and Fe-ion-dependent rapid proliferation than those of strains derived from patients without IDA.

MATERIALS AND METHODS:

Twenty-four H. pylori strains from IDA patients (IDA strains) and 25 strains from patients who had H. pylori gastritis without anemia (non-IDA strains) were examined. Their nucleotide sequences of napA, fur, and feoB, which contribute to Fe ion uptake, were determined.

RESULTS:

Numerous polymorphisms of the three genes were found in both strains. Frequency of neutrophil-activating protein A (NapA), which encoded by napA, with threonine at amino acid residue No. 70 (Thr70-type NapA) was significantly higher in IDA strains than in non-IDA strains. Strains with Thr70-type NapA showed significantly higher levels of Fe(3+) and Fe(2+) uptake than did strains with other types, Ser70-type of NapA, which is found in standard strains. Other significantly different occurrences of polymorphisms between IDA and non-IDA groups were not observed in these genes.

CONCLUSION:

The results suggest that H. pylori strains with Thr70-type NapA have enhanced Fe ion uptake ability and are associated with the pathogenesis of IDA.

• Authors: Shin-Ichi Yokota, Nariaki Toita, Soh Yamamoto, Nobuhiro Fujii, Mutsuko Konno
• Journal: Helicobacter (Helicobacter) Vol. 18 Issue 2 Pg. 112-6 (Apr 2013) ISSN: 1523-5378 [Electronic] England
• PMID: 23067298 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2012 Blackwell Publishing Ltd.

Chemical References

• Bacterial Proteins
• neutrophil-activating protein A, Helicobacter pylori
• Iron

Topics

• Amino Acid Sequence
• Anemia, Iron-Deficiency (complications, genetics, immunology, virology)
• Bacterial Proteins (genetics)
• Female
• Helicobacter Infections (complications, genetics, immunology)
• Helicobacter pylori (genetics, metabolism)
• Humans
• Iron (metabolism)
• Male
• Molecular Sequence Data
• Neutrophils (immunology)
• Polymorphism, Genetic
• Sequence Analysis, DNA