T-cell receptor repertoires of tumor-infiltrating lymphocytes after hyperthermia using functionalized magnetite nanoparticles.

Abstract

AIM:

Accumulating evidence has indicated that hyperthermia using magnetite nanoparticles induces antitumor immunity. This study investigated the diversity of T-cell receptors (TCRs) in tumor-infiltrating lymphocytes after hyperthermia using magnetite nanoparticles.

MATERIALS & METHODS:

Functionalized magnetite nanoparticles, N-propionyl-4-S-cysteaminylphenol (NPrCAP)/magnetite, were synthesized by conjugating the melanogenesis substrate NPrCAP with magnetite nanoparticles. NPrCAP/magnetite nanoparticles were injected into B16 melanomas in C57BL/6 mice, which were subjected to an alternating magnetic field for hyperthermia treatment.

RESULTS:

Enlargement of the tumor-draining lymph nodes was observed after hyperthermia. The TCR repertoire was restricted in tumor-infiltrating lymphocytes, and expansion of Vβ11(+) T cells was preferentially found. DNA sequences of the third complementaritydetermining regions revealed the presence of clonally expanded T cells.

CONCLUSION:

These results indicate that the T-cell response in B16 melanomas after hyperthermia is dominated by T cells directed toward a limited number of epitopes and that epitope-specific T cells frequently use a restricted TCR repertoire.

• Authors: Akira Ito, Masaki Yamaguchi, Noriaki Okamoto, Yuji Sanematsu, Yoshinori Kawabe, Kazumasa Wakamatsu, Shosuke Ito, Hiroyuki Honda, Takeshi Kobayashi, Eiichi Nakayama, Yasuaki Tamura, Masae Okura, Toshiharu Yamashita, Kowichi Jimbow, Masamichi Kamihira
• Journal: Nanomedicine (London, England) (Nanomedicine (Lond)) Vol. 8 Issue 6 Pg. 891-902 (Jun 2013) ISSN: 1748-6963 [Electronic] England
• PMID: 23066648 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Magnetite Nanoparticles
• Receptors, Antigen, T-Cell

Topics

• Animals
• Female
• Hyperthermia, Induced (methods)
• Lymph Nodes (immunology, pathology)
• Lymphocytes, Tumor-Infiltrating (immunology, pathology)
• Magnetic Fields
• Magnetite Nanoparticles (chemistry, therapeutic use)
• Melanoma, Experimental (immunology, pathology, therapy)
• Mice
• Mice, Inbred C57BL
• Receptors, Antigen, T-Cell (immunology)
• T-Lymphocytes (immunology, pathology)