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Involvement of Ext1 and heparanase in migration of mouse FBJ osteosarcoma cells.

Abstract
To know the involvement of glycosaminoglycans (GAGs) in the metastasis of mouse FBJ osteosarcoma cells, N(α)-lauroyl-O-(β-D-xylopyranosyl)-L-serinamide (Xyl-Ser-C12), which initiates elongation of GAG chains using the glycan biosynthesis system in cells, was administered to FBJ cells with different metastatic capacities. Production of glycosylated products derived from Xyl-Ser-C12, especially heparan sulfate (HS) GAG-type oligosaccharides such as GalNAc-GlcA-GlcNAc-GlcA-Gal-Gal-Xyl-Ser-C12, was indicated in poorly metastatic FBJ-S1 cells more than in highly metastatic FBJ-LL cells by LC-MS. The results of RT-PCR revealed that HS synthases, Ext1 and Ext2, were expressed in FBJ-S1 cells more than in FBJ-LL cells. Furthermore, siRNA against Ext1 suppressed the expression of HS and enhanced the motility of FBJ-S1 cells. In addition, the expression of heparanase (HPSE) was enhanced in Ext-1-knockdown FBJ-S1 cells, and responsible for the increase in cell motility caused by the down-regulation of Ext1 expression. Our data provide the first evidence that Ext1 regulates the expression of HPSE and also indicated that levels of Ext1 and HPSE influenced the motility of FBJ cells.
AuthorsYinan Wang, XiaoYan Yang, Sadako Yamagata, Tatsuya Yamagata, Toshinori Sato
JournalMolecular and cellular biochemistry (Mol Cell Biochem) Vol. 373 Issue 1-2 Pg. 63-72 (Jan 2013) ISSN: 1573-4919 [Electronic] Netherlands
PMID23054193 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • RNA, Small Interfering
  • Heparitin Sulfate
  • N-Acetylglucosaminyltransferases
  • exostosin-1
  • exostosin-2
  • heparanase
  • Glucuronidase
Topics
  • Animals
  • Carbohydrate Sequence
  • Cell Line, Tumor
  • Cell Movement
  • Gene Expression
  • Gene Knockdown Techniques
  • Glucuronidase (genetics, metabolism)
  • Heparitin Sulfate (biosynthesis)
  • Mice
  • Molecular Sequence Data
  • N-Acetylglucosaminyltransferases (genetics, metabolism)
  • Osteosarcoma
  • RNA, Small Interfering (genetics)

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