Abstract | AIMS: MAIN METHODS: Studies were performed in 18 conscious dogs, chronically instrumented to measure coronary blood flow and myocardial wall thickening (WT). In Group 1 (control; n=7), a 10-min coronary occlusion was produced followed by reperfusion; WT was monitored until full recovery. In Group 2 (n=6), the same occlusion-reperfusion protocol was performed 24h after inhalation of 1 MAC isoflurane (1.4% in O(2)) for 60 min. In Group 3 (n=5), the late anti-stunning effect of isoflurane was evaluated following non-selective inhibition of NOS with N-nitro- l-arginine (l-NA, 30 mg/kg on 3 days beginning 1 day prior to isoflurane). Expression of eNOS and iNOS protein was measured by Western blotting. KEY FINDINGS: Two to 3h of reperfusion was required for recovery of WT following isoflurane (Group 2). In contrast, without isoflurane (Group 1), WT remained markedly reduced (30% below baseline) at this time point and required more than 6h of reperfusion for recovery. Treatment with l-NA (Group 3) did not alter time-course of recovery of WT following isoflurane. Isoflurane caused an increased expression of eNOS, but not of iNOS. SIGNIFICANCE:
Isoflurane produced late preconditioning against myocardial stunning. Although this effect was associated with an up-regulation of eNOS, its persistence following l-NA suggested that an increased production of nitric oxide did not play an obligatory role.
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Authors | Song-Jung Kim, Gautam Malik, Maged M Saad, Sung-Ho Yoon, Joaquin B Gonzalez, George J Crystal |
Journal | Life sciences
(Life Sci)
Vol. 91
Issue 23-24
Pg. 1201-6
(Dec 10 2012)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 23044225
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Elsevier Inc. All rights reserved. |
Chemical References |
- Nitroarginine
- Nitric Oxide
- Isoflurane
- Nitric Oxide Synthase Type II
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Topics |
- Animals
- Blood Flow Velocity
- Blotting, Western
- Dogs
- Ischemic Preconditioning, Myocardial
(methods)
- Isoflurane
(pharmacology)
- Myocardial Stunning
(prevention & control)
- Nitric Oxide
(metabolism)
- Nitric Oxide Synthase Type II
(antagonists & inhibitors)
- Nitroarginine
(pharmacology)
- Reperfusion
(methods)
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