Microcirculatory effects of selective receptor blockade during hemorrhagic shock treatment with vasopressin: experimental study in the hamster dorsal chamber.

Abstract

Hemorrhagic shock is a major cause of death in modern societies. Some patients, when treated, fail to sustain normal cardiovascular parameters, requiring fluid therapy and vasoactive drugs. Among drugs with cardiovascular profile other than catecholamine, vasopressin (VP) is emerging as an option. To better understand its effects during hemorrhagic shock, we compared the effects of VP and noradrenaline (NA), associated to fluid therapy. In this work, hamsters were subjected to shock by withdrawal of 40% of their blood volume and were then divided into five groups. One group was treated with saline solution, and the remaining ones with VP (three groups) and NA (one group) combined to fluid resuscitation. To assess receptor role, two more VP groups were pretreated with specific receptor blockers (anti-V1 or anti-V2, respectively) before its infusion. Microcirculatory parameters such as vessel diameter, red blood cell velocity, and functional capillary density were evaluated. In addition, blood gas analysis and lactate levels were also determined. Measurements were performed at baseline, after shock, and after treatment. At the end, leukocyte-endothelium interaction was evaluated, and animals were followed up to determine survival time. Neither saline solution nor NA recovered microcirculatory parameters, but VP treatment returned to near baseline values, except when V2 receptors were blocked. Functional capillary density was higher in the VP group after treatment, without statistical difference from baseline values. When V2 receptors were blocked, recovery was not achieved after treatment. The VP group also had a smaller number of adhering leukocytes and improved 72-h survival time compared with the NA one. This study suggests that, in hemorrhagic shock, treatment with low-dose VP, in combination with fluid therapy, improves tissue perfusion. This outcome is mediated mostly by V2 receptors, eliciting vasodilatation and consequently blood flow redistribution through the microcirculation.

• Authors: Ronald Lima, Nivaldo R Villela, Eliete Bouskela
• Journal: Shock (Augusta, Ga.) (Shock) Vol. 38 Issue 5 Pg. 493-8 (Nov 2012) ISSN: 1540-0514 [Electronic] United States
• PMID: 23042188 (Publication Type: Journal Article)

Chemical References

• Antibodies
• Antidiuretic Hormone Receptor Antagonists
• Vasoconstrictor Agents
• Vasopressins

Topics

• Animals
• Antibodies (pharmacology)
• Antidiuretic Hormone Receptor Antagonists
• Blood Flow Velocity (drug effects)
• Cricetinae
• Disease Models, Animal
• Female
• Humans
• Male
• Mesocricetus
• Microcirculation (drug effects)
• Shock, Hemorrhagic (drug therapy, physiopathology)
• Vasoconstrictor Agents (pharmacology)
• Vasopressins (pharmacology)