Abstract |
Successful regeneration and remodeling of neuromuscular junctions are critical for restoring functional capacities and properties of skeletal muscle after damage, and axon-guidance molecules may be involved in the signaling that regulates such restoration. Recently, we found that early-differentiated satellite cells up-regulate a secreted neural chemorepellent Sema3A upon in vivo muscle- crush injury. The study also revealed that Sema3A expression is up-regulated in primary satellite-cell cultures in response to hepatocyte growth factor (HGF) and basic fibroblast growth factor ( FGF2) and is prevented by transforming growth factor (TGF)-β2, 3. In order to verify the physiological significance of this regulation in vitro, the present study was designed to estimate the time-course of extracellular HGF, FGF2 and TGF-β3 concentrations after crush-injury of Gastrocnemius muscle in the rat lower hind-limb, using a combination of a non-homogenization/non-spin extraction of extracellular wound fluids and enhanced chemiluminescence-Western blotting analyses. Results clearly demonstrated that active HGF and FGF2 are prevalent in 2-8 days post-crush, whereas active TGF-β3 increases after 12 days, providing a better understanding of the time-coordinated levels of HGF, FGF2 and TGF-β3 that drive regulation of Sema3A expression during regenerative intramuscular moto-neuritogenesis.
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Authors | Mai-Khoi Q DO, Takahiro Suzuki, Borjigin Gerelt, Yusuke Sato, Wataru Mizunoya, Mako Nakamura, Yoshihide Ikeuchi, Judy E Anderson, Ryuichi Tatsumi |
Journal | Animal science journal = Nihon chikusan Gakkaiho
(Anim Sci J)
Vol. 83
Issue 10
Pg. 712-7
(Oct 2012)
ISSN: 1740-0929 [Electronic] Australia |
PMID | 23035711
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2012 Japanese Society of Animal Science. |
Chemical References |
- Semaphorin-3A
- Transforming Growth Factor beta3
- Fibroblast Growth Factors
- Hepatocyte Growth Factor
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Topics |
- Animals
- Blotting, Western
- Fibroblast Growth Factors
(analysis)
- Hepatocyte Growth Factor
(analysis)
- Muscle, Skeletal
(injuries)
- Rats
- Semaphorin-3A
(metabolism)
- Time Factors
- Transforming Growth Factor beta3
(analysis)
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