HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-β3 in crush-injured skeletal muscle.

Abstract
Successful regeneration and remodeling of neuromuscular junctions are critical for restoring functional capacities and properties of skeletal muscle after damage, and axon-guidance molecules may be involved in the signaling that regulates such restoration. Recently, we found that early-differentiated satellite cells up-regulate a secreted neural chemorepellent Sema3A upon in vivo muscle-crush injury. The study also revealed that Sema3A expression is up-regulated in primary satellite-cell cultures in response to hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) and is prevented by transforming growth factor (TGF)-β2, 3. In order to verify the physiological significance of this regulation in vitro, the present study was designed to estimate the time-course of extracellular HGF, FGF2 and TGF-β3 concentrations after crush-injury of Gastrocnemius muscle in the rat lower hind-limb, using a combination of a non-homogenization/non-spin extraction of extracellular wound fluids and enhanced chemiluminescence-Western blotting analyses. Results clearly demonstrated that active HGF and FGF2 are prevalent in 2-8 days post-crush, whereas active TGF-β3 increases after 12 days, providing a better understanding of the time-coordinated levels of HGF, FGF2 and TGF-β3 that drive regulation of Sema3A expression during regenerative intramuscular moto-neuritogenesis.
AuthorsMai-Khoi Q DO, Takahiro Suzuki, Borjigin Gerelt, Yusuke Sato, Wataru Mizunoya, Mako Nakamura, Yoshihide Ikeuchi, Judy E Anderson, Ryuichi Tatsumi
JournalAnimal science journal = Nihon chikusan Gakkaiho (Anim Sci J) Vol. 83 Issue 10 Pg. 712-7 (Oct 2012) ISSN: 1740-0929 [Electronic] Australia
PMID23035711 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2012 Japanese Society of Animal Science.
Chemical References
  • Semaphorin-3A
  • Transforming Growth Factor beta3
  • Fibroblast Growth Factors
  • Hepatocyte Growth Factor
Topics
  • Animals
  • Blotting, Western
  • Fibroblast Growth Factors (analysis)
  • Hepatocyte Growth Factor (analysis)
  • Muscle, Skeletal (injuries)
  • Rats
  • Semaphorin-3A (metabolism)
  • Time Factors
  • Transforming Growth Factor beta3 (analysis)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: