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Enteropathogenic e.coli sustains iodoacetamide-induced ulcerative colitis-like colitis in rats: modulation of IL-1β, IL-6, TNF-α, COX-2, and apoptosisi.

Abstract
Pathogenic or non-pathogenic bacteria from flora may play a key role in inflammatory bowel disease (IBD) pathogenesis. However, a specific infectious agent causing IBD has not been identified. This study assessed the impact of enteropathogenic E. coli (EPEC) on the modulation of IL-1beta, IL-6, TNF- alpha, COX-2, BAX and Bcl-2 expression, in sustaining inflammation of a rat colitis model. Two hundred male Sprague-Dawley rats (4 groups) were inoculated weekly or bi-weekly for 70 days, with 1 percent methylcellulose (MC), (b) 6 percent iodoacetamide (IA) in 1 percent MC, (c) 4x108 CFU of EPEC, and (d) IA+EPEC. After a month, treatment was stopped in half of the animals in each group. IL-1beta, IL-6, TNF-alpha, COX-2, BAX and Bcl-2 expression were measured in colonic mucosa scrapings. IL-1beta, IL-6, TNF-alpha, and COX-2 were significantly increased in colonic mucosa of the IA+EPEC group and to a lesser but significant level in the IA group compared to controls, or EPEC alone, both in continued and discontinued treatment groups. Additionally, the BAX/Bcl-2 ratio decreased, indicating less apoptosis in the IA+EPEC group which exhibited more necrosis. These effects increased with experiment duration. This work provides new arguments favouring the role of bacteria in IBD pathogenesis.
AuthorsI Abdallah Hajj Hussein, J N Freund, J M Reimund, A Shams, M Yamine, A Leone, A R Jurjus
JournalJournal of biological regulators and homeostatic agents (J Biol Regul Homeost Agents) 2012 Jul-Sep Vol. 26 Issue 3 Pg. 515-26 ISSN: 0393-974X [Print] Italy
PMID23034271 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Alkylating Agents
  • Bax protein, rat
  • Interleukin-1beta
  • Interleukin-6
  • Proto-Oncogene Proteins c-bcl-2
  • Tumor Necrosis Factor-alpha
  • bcl-2-Associated X Protein
  • Cyclooxygenase 2
  • Ptgs2 protein, rat
  • Iodoacetamide
Topics
  • Alkylating Agents (adverse effects, pharmacology)
  • Animals
  • Apoptosis (drug effects)
  • Colitis, Ulcerative (chemically induced, metabolism, microbiology, pathology)
  • Colon (metabolism, microbiology, pathology)
  • Cyclooxygenase 2 (biosynthesis)
  • Enteropathogenic Escherichia coli
  • Escherichia coli Infections (chemically induced, metabolism, microbiology, pathology)
  • Gene Expression Regulation (drug effects)
  • Interleukin-1beta (biosynthesis)
  • Interleukin-6 (biosynthesis)
  • Intestinal Mucosa (metabolism, microbiology, pathology)
  • Iodoacetamide (adverse effects, pharmacology)
  • Male
  • Proto-Oncogene Proteins c-bcl-2 (biosynthesis)
  • Rats
  • Rats, Sprague-Dawley
  • Tumor Necrosis Factor-alpha (biosynthesis)
  • bcl-2-Associated X Protein (biosynthesis)

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