Abstract | BACKGROUND:
Liposomes containing pH-sensitive polymers are promising candidates for the treatment of tumors and localized infection. This study aimed to identify parameters influencing the extent of contents release from poly(ethylacrylic acid) ( PEAA) vesicles, focusing on the effects of polymer size, lipid composition, vesicle surface charge, and temperature. METHODS: RESULTS: The release efficiency of the calcein-entrapped vesicles was found to be dependent on the PEAA polymer size. PEAA vesicles containing a phosphatidylcholine to cholesterol ratio of 60:40 (mol/mol) released more than 80% of their calcein content when the molecular weight of PEAA was larger than 8.4 kDa. Therefore, the same-sized polymer of 8.4 kDa was used for the rest of study. The calcein release potential was found to decrease as the percentage of cholesterol increased and with an increase in the phosphocholine acyl chain length ( DMPC DPPC DSPC). Negatively charged and neutral vesicles released similar amounts of calcein, whereas positively charged liposomes released a significant amount of their contents. pH-sensitive release was dependent on temperature. Dramatic content release was observed at higher temperatures. CONCLUSION: The observed synergistic effect of pH and temperature on release of the contents of PEAA vesicles suggests that this pH-sensitive liposome might be a good candidate for intracellular drug delivery in the treatment of tumors or localized infection.
|
Authors | Tingli Lu, Zhao Wang, Yufan Ma, Yang Zhang, Tao Chen |
Journal | International journal of nanomedicine
(Int J Nanomedicine)
Vol. 7
Pg. 4917-26
( 2012)
ISSN: 1178-2013 [Electronic] New Zealand |
PMID | 23028220
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Acrylates
- Delayed-Action Preparations
- Lipids
- Liposomes
- Nanocapsules
- poly(2-ethylacrylic acid)
|
Topics |
- Absorption
- Acrylates
(chemistry)
- Delayed-Action Preparations
(chemistry)
- Hydrogen-Ion Concentration
- Lipids
(chemistry)
- Liposomes
(chemistry)
- Nanocapsules
(chemistry, ultrastructure)
- Particle Size
- Permeability
- Static Electricity
- Surface Properties
- Temperature
|