Abstract |
CD4(+) Th2 cytokine responses promote the development of allergic inflammation and are critical for immunity to parasitic helminth infection. Recent studies highlighted that basophils can promote Th2 cytokine-mediated inflammation and that phenotypic and functional heterogeneity exists between classical IL-3-elicited basophils and thymic stromal lymphopoietin (TSLP)-elicited basophils. However, whether distinct basophil populations develop after helminth infection and their relative contributions to anti-helminth immune responses remain to be defined. After Trichinella spiralis infection of mice, we show that basophil responses are rapidly induced in multiple tissue compartments, including intestinal-draining lymph nodes. Trichinella-induced basophil responses were IL-3-IL-3R independent but critically dependent on TSLP-TSLPR interactions. Selective depletion of basophils after Trichinella infection impaired infection-induced CD4(+) Th2 cytokine responses, suggesting that TSLP-dependent basophils augment Th2 cytokine responses after helminth infection. The identification and functional classification of TSLP-dependent basophils in a helminth infection model, coupled with their recently described role in promoting atopic dermatitis, suggests that these cells may be a critical population in promoting Th2 cytokine-associated inflammation in a variety of inflammatory or infectious settings. Collectively, these data suggest that the TSLP-basophil pathway may represent a new target in the design of therapeutic intervention strategies to promote or limit Th2 cytokine-dependent immunity and inflammation.
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Authors | Paul R Giacomin, Mark C Siracusa, Kevin P Walsh, Richard K Grencis, Masato Kubo, Michael R Comeau, David Artis |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 189
Issue 9
Pg. 4371-8
(Nov 01 2012)
ISSN: 1550-6606 [Electronic] United States |
PMID | 23024277
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Thymic Stromal Lymphopoietin
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Topics |
- Animals
- Basophils
(immunology, parasitology, pathology)
- Cytokines
(biosynthesis, physiology)
- Inflammation
(immunology, metabolism, parasitology)
- Intestinal Mucosa
(immunology, parasitology, pathology)
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Mice, Transgenic
- Stromal Cells
(immunology, parasitology, pathology)
- Th2 Cells
(immunology, parasitology, pathology)
- Thymus Gland
(immunology, parasitology, pathology)
- Trichinella spiralis
(immunology)
- Trichinellosis
(immunology, metabolism, pathology)
- Thymic Stromal Lymphopoietin
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