[Effect of artemisinin on the expressions of GRalpha mRNA, GRbeta mRNA and P300/CBP protein in lupus nephritis mice].

Abstract

OBJECTIVE:

To investigate the effects of Artemisinin on the expressions of GRalpha mRNA, GRbeta mRNA and P300/CBP protein in lupus nephritis mice.

METHODS:

Forty hybrid female mice were randomly and equally divided into four groups with the method of random number table: control group, model group, prednisone group administrated with 6.45 mg/kg Artemisinin (Art) suspension. A mice model of LN was established by injection with living lymph cell suspension. The expressions of GC receptor alpha (GRalpha) mRNA, GC receptor beta (GRbeta) mRNA in peripheral blood mononuclear cells (PBMCs), and transcriptional coactivator P300/CBP protein in renal tissue were respectively measured by the technique of RT - PCR and immunohistochemical assay.

RESULTS:

Compared with the model group. The expression of transcriptional coactivator P300/CBP protein in renal tissue and GRa mRNA in PBMCs of treatment groups was increased significantly, GRbeta mRNA expression was significantly decreased (P < 0.05 or P < 0.01). And the Art group had a better effect on the expression of GRalpha mRNA and transcriptional coactivator P300/CBP protein than that of the prednisone group (P < 0.01).

CONCLUSION:

The underlying therapeutic mechanism may be correlated with the regulation of Art on the expressions of GRalpha mRNA, GRbeta mRNA in PBMC and transcriptional coactivator P300/CBP protein in renal tissue.

• Authors: Xi-Li Wu, Wan-Sen Sun, Xing-Min Shi, Zhu Wang, Peng An, Cheng-Lin Qiao
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 35 Issue 4 Pg. 608-12 (Apr 2012) ISSN: 1001-4454 [Print] China
• PMID: 23019910 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Artemisinins
• RNA, Messenger
• Receptors, Glucocorticoid
• glucocorticoid receptor alpha
• glucocorticoid receptor beta
• artemisinin
• p300-CBP Transcription Factors
• Prednisone

Topics

• Animals
• Artemisia annua (chemistry)
• Artemisinins (administration & dosage, pharmacology)
• Disease Models, Animal
• Female
• Leukocytes, Mononuclear (drug effects, metabolism)
• Lupus Nephritis (drug therapy, metabolism)
• Mice
• Mice, Inbred Strains
• Prednisone (administration & dosage, pharmacology)
• RNA, Messenger (genetics, metabolism)
• Receptors, Glucocorticoid (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• p300-CBP Transcription Factors (genetics, metabolism)