Der p 2 recombinant bacille Calmette-Guérin targets dendritic cells to inhibit allergic airway inflammation in a mouse model of asthma.

Abstract

BACKGROUND:

Previous studies showed that a recombinant bacille Calmette-Guérin (rBCG) which expressed the Der p 2 of house dust mites (Der p 2 rBCG) could suppress asthmatic airway inflammation. There are two possible mechanisms: (1) Der p 2 rBCG elicits immune deviation from Th2 to Th1, and (2) Der p 2 rBCG induces antigen-specific regulatory T cells. However, the role of dendritic cell (DC) Der p 2 rBCG in this protective effect and in reprogramming T-cell commitment still needs to be studied.

OBJECTIVES:

The aim of this study was to determine whether DCs play a central role in the Der p 2 rBCG-mediated inhibition of allergic airway inflammation.

METHODS:

DCs were collected from Der p 2 rBCG-immunized mice (Der p 2 rBCG-DCs) and adoptively transferred to Der p 2-sensitized mice. The effects of DCs on airway inflammation and immune regulation were analyzed.

RESULTS:

Adoptive transfer of DCs from Der p 2 rBCG-immunized mice suppressed asthmatic responses, including airway inflammation, mucin secretion and airway responsiveness. Der p 2 rBCG-DCs could effectively inhibit excessive Th2 immune responses and induced a subtype of CD4+CD25+Foxp3+ anti-specific regulatory T cells in this asthma model. Furthermore, Der p 2 rBCG immunization recruited more plasmacytoid DCs in abdominal draining lymph nodes.

CONCLUSIONS:

These findings suggest that DCs played a key role in Der p 2 rBCG-induced immunoregulation. Compared with BCG, Der p 2 rBCG displayed a more potent inhibitory effect on asthma responses, which may be related to the increase in plasmacytoid DC recruitment. These results improve our understanding of the cellular basis of Der p 2 BCG-mediated inhibition of asthma.

• Authors: Shuo-Yao Qu, Hai-Feng Ou-Yang, Ya-Long He, Qi Wan, Jie-Ran Shi, Chang-Gui Wu
• Journal: Respiration; international review of thoracic diseases (Respiration) Vol. 85 Issue 1 Pg. 49-58 ( 2013) ISSN: 1423-0356 [Electronic] Switzerland
• PMID: 23018133 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 S. Karger AG, Basel.

Chemical References

• Antigens, Dermatophagoides
• Arthropod Proteins
• BCG Vaccine
• Dermatophagoides pteronyssinus antigen p 2
• Recombinant Fusion Proteins

Topics

• Animals
• Antigens, Dermatophagoides (immunology, therapeutic use)
• Arthropod Proteins (immunology, therapeutic use)
• Asthma (immunology, pathology, therapy)
• BCG Vaccine (immunology, therapeutic use)
• Dendritic Cells (immunology)
• Disease Models, Animal
• Female
• Immunotherapy, Adoptive (methods)
• Inflammation (drug therapy, immunology, pathology)
• Mice
• Mice, Inbred C57BL
• Recombinant Fusion Proteins (immunology, therapeutic use)
• Th1 Cells (immunology)
• Th2 Cells (immunology)