Abstract | OBJECTIVE: METHODS: On the established human pancreatic cancer cells Panc-1-XS, the expression of XIAP and survivin was inhibited simultaneously. Cell invasion and migration were detected by Transwell chamber experiments and scratch test, and the expression of epithelial marker E-cadherin, mesenchymal markers Slug, phosphatase and tensin homolog deleted on chromosome ten (PTEN) and P-Akt protein was determined by Western blot. RESULTS: Cell invasion and migration of Panc-1-XS cells decreased significantly, accompanied by significantly upregulated protein expression of E-cadherin, and significantly declined protein expression of the Slug, indicating increased mesenchymal-epithelial conversion (MET); and increased protein expression of PTEN, and declined protein expression of P-Akt. CONCLUSION: Simultaneously inhibiting the expression of XIAP and survivin can partially reverse EMT phenotype of pancreatic cancer Panc-1 cells, which then significantly reduces the cell invasion and migration of Panc-1 cell lines. This process may be regulated by PTEN/PI3K/Akt signaling pathway.
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Authors | Hongyan Zai, Xiaoping Yi, Yixiong Li, Chun Jiang, Xinsheng Lu |
Journal | Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
(Zhong Nan Da Xue Xue Bao Yi Xue Ban)
Vol. 37
Issue 9
Pg. 883-8
(Sep 2012)
ISSN: 1672-7347 [Print] China |
PMID | 23000761
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, CD
- BIRC5 protein, human
- CDH1 protein, human
- Cadherins
- Inhibitor of Apoptosis Proteins
- SNAI1 protein, human
- Snail Family Transcription Factors
- Survivin
- Transcription Factors
- X-Linked Inhibitor of Apoptosis Protein
- XIAP protein, human
- Proto-Oncogene Proteins c-akt
- PTEN Phosphohydrolase
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Topics |
- Antigens, CD
- Cadherins
(metabolism)
- Cell Line, Tumor
- Cell Movement
- Epithelial-Mesenchymal Transition
(genetics)
- Humans
- Inhibitor of Apoptosis Proteins
(genetics, metabolism)
- Neoplasm Invasiveness
- PTEN Phosphohydrolase
(metabolism)
- Pancreatic Neoplasms
(pathology)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Signal Transduction
- Snail Family Transcription Factors
- Survivin
- Transcription Factors
(metabolism)
- X-Linked Inhibitor of Apoptosis Protein
(genetics, metabolism)
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