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[Simultaneous inhibition of XIAP and survivin expression on EMT and invasion of human pancreatic cancer cells].

AbstractOBJECTIVE:
To investigate the simultaneous inhibition of X-linked inhibitor of apoptosis protein (XIAP) and survivin expression on epithelial-mesenchymal transition (EMT) and invasiion of pancreatic cancer cells Panc-1, and its mechanism.
METHODS:
On the established human pancreatic cancer cells Panc-1-XS, the expression of XIAP and survivin was inhibited simultaneously. Cell invasion and migration were detected by Transwell chamber experiments and scratch test, and the expression of epithelial marker E-cadherin, mesenchymal markers Slug, phosphatase and tensin homolog deleted on chromosome ten (PTEN) and P-Akt protein was determined by Western blot.
RESULTS:
Cell invasion and migration of Panc-1-XS cells decreased significantly, accompanied by significantly upregulated protein expression of E-cadherin, and significantly declined protein expression of the Slug, indicating increased mesenchymal-epithelial conversion (MET); and increased protein expression of PTEN, and declined protein expression of P-Akt.
CONCLUSION:
Simultaneously inhibiting the expression of XIAP and survivin can partially reverse EMT phenotype of pancreatic cancer Panc-1 cells, which then significantly reduces the cell invasion and migration of Panc-1 cell lines. This process may be regulated by PTEN/PI3K/Akt signaling pathway.
AuthorsHongyan Zai, Xiaoping Yi, Yixiong Li, Chun Jiang, Xinsheng Lu
JournalZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 37 Issue 9 Pg. 883-8 (Sep 2012) ISSN: 1672-7347 [Print] China
PMID23000761 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, CD
  • BIRC5 protein, human
  • CDH1 protein, human
  • Cadherins
  • Inhibitor of Apoptosis Proteins
  • SNAI1 protein, human
  • Snail Family Transcription Factors
  • Survivin
  • Transcription Factors
  • X-Linked Inhibitor of Apoptosis Protein
  • XIAP protein, human
  • Proto-Oncogene Proteins c-akt
  • PTEN Phosphohydrolase
Topics
  • Antigens, CD
  • Cadherins (metabolism)
  • Cell Line, Tumor
  • Cell Movement
  • Epithelial-Mesenchymal Transition (genetics)
  • Humans
  • Inhibitor of Apoptosis Proteins (genetics, metabolism)
  • Neoplasm Invasiveness
  • PTEN Phosphohydrolase (metabolism)
  • Pancreatic Neoplasms (pathology)
  • Proto-Oncogene Proteins c-akt (metabolism)
  • Signal Transduction
  • Snail Family Transcription Factors
  • Survivin
  • Transcription Factors (metabolism)
  • X-Linked Inhibitor of Apoptosis Protein (genetics, metabolism)

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