Microemulsion-based oxyresveratrol for topical treatment of herpes simplex virus (HSV) infection: physicochemical properties and efficacy in cutaneous HSV-1 infection in mice.

Abstract

The physicochemical properties of the optimized microemulsion and the permeating ability of oxyresveratrol in microemulsion were evaluated, and the efficacy of oxyresveratrol microemulsion in cutaneous herpes simplex virus type 1 (HSV-1) infection in mice was examined. The optimized microemulsion was composed of 10% w/w of isopropyl myristate, 35% w/w of Tween 80, 35% w/w of isopropyl alcohol, and 20% w/w of water. The mean particle diameter was 9.67 ± 0.58 nm, and the solubility of oxyresveratrol in the microemulsion was 196.34 ± 0.80 mg/ml. After accelerated and long-term stability testing, the microemulsion base and oxyresveratrol-loaded microemulsion were stable. The cumulative amount of oxyresveratrol permeating through shed snake skin from microemulsion at 6 h was 93.04 times compared to that of oxyresveratrol from Vaseline, determined at 20% w/w concentration. In cutaneous HSV-1 infection in mice, oxyresveratrol microemulsion at 20%, 25%, and 30% w/w, topically applied five times daily for 7 days after infection, was significantly effective in delaying the development of skin lesions and protecting from death (p < 0.05) compared with the untreated control. Oxyresveratrol microemulsion at 25% and 30% w/w was significantly more effective than that of 30% w/w of oxyresveratrol in Vaseline (p < 0.05) and was as effective as 5% w/w of acyclovir cream, topically applied five times daily (p > 0.05). These results demonstrated that topical oxyresveratrol microemulsion at 20-30% w/w was suitable for cutaneous HSV-1 mouse infection.

• Authors: Pattaraporn Sasivimolphan, Vimolmas Lipipun, Garnpimol Ritthidej, Khanidtha Chitphet, Yoshihiro Yoshida, Tohru Daikoku, Boonchoo Sritularak, Kittisak Likhitwitayawuid, Pornpen Pramyothin, Masao Hattori, Kimiyasu Shiraki
• Journal: AAPS PharmSciTech (AAPS PharmSciTech) Vol. 13 Issue 4 Pg. 1266-75 (Dec 2012) ISSN: 1530-9932 [Electronic] United States
• PMID: 22996670 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Agents
• Emulsions
• Plant Extracts
• Stilbenes
• puag-haad
• Petrolatum
• Acyclovir

Topics

• Acyclovir (administration & dosage)
• Administration, Topical
• Animals
• Antiviral Agents (administration & dosage, chemistry)
• Chlorocebus aethiops
• Drug Stability
• Emulsions (administration & dosage, chemistry)
• Female
• Herpes Simplex (drug therapy, virology)
• Herpesvirus 1, Human (isolation & purification)
• Mice
• Mice, Inbred BALB C
• Particle Size
• Permeability
• Petrolatum (administration & dosage)
• Plant Extracts (administration & dosage, chemistry)
• Skin (drug effects, metabolism)
• Skin Cream (administration & dosage, chemistry)
• Skin Diseases, Viral (drug therapy, virology)
• Snakes (metabolism)
• Solubility
• Stilbenes (administration & dosage, chemistry)
• Vero Cells