Abstract | BACKGROUND: MATERIALS AND METHODS: We injected Pseudomonas aeruginosa or lipopolysaccharide in the backs of wild-type, Tnfrsf1a(-/-) (deficient of TNF-α receptor 1), and TLR4(-/-) mice at 8 h after 30% total body surface area burn. The animals were sacrificed at 16 h after burn and lung tissues were harvested and examined for determining pulmonary microvascular dysfunction and interleukin (IL)-1β, iNOS, and TLR4 expression. The blood of animals was harvested for bacterial count assay. The effect of S-methylisothiourea, an iNOS inhibitor, on P aeruginosa infection with thermal injury pretreatment-induced lung damage was also examined. RESULTS: P aeruginosa or lipopolysaccharide injection with thermal injury pretreatment enhanced TLR4, iNOS, and IL-1β expression and pulmonary microvascular dysfunction in Tnfrsf1a(-/-) mice compared with wild-type mice. P aeruginosa infection with thermal injury pretreatment did not induce IL-1β or iNOS expression and mortality in TLR4(-/-) mice. S-methylisothiourea treatment significantly decreased P aeruginosa infection with thermal injury pretreatment-induced lung injury, blood bacterial counts, pulmonary IL-1β expression, and mortality in Tnfrsf1a(-/-) mice. CONCLUSIONS: Given that absence of the TNF-α receptor 1 is associated with increased lung permeability, we conclude that TNF-α decreases P aeruginosa infection-induced lung damage in burn mice through negative regulation of TLR4 as well as iNOS expression, and iNOS inhibitor might be useful in reversing anti-TNF-α therapy-induced lung injury in burn.
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Authors | Tzyy-Bin Tsay, Ming-Chieh Yang, Pei-Hsuan Chen, Cheng-Ta Lin, Ching-Mei Hsu, Lee-Wei Chen |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 179
Issue 1
Pg. 106-14
(Jan 2013)
ISSN: 1095-8673 [Electronic] United States |
PMID | 22995661
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Inc. All rights reserved. |
Chemical References |
- Enzyme Inhibitors
- Interleukin-1beta
- Lipopolysaccharides
- Receptors, Tumor Necrosis Factor, Type I
- Tlr4 protein, mouse
- Tnfrsf1a protein, mouse
- Toll-Like Receptor 4
- Tumor Necrosis Factor-alpha
- Isothiuronium
- Nitric Oxide Synthase Type II
- S-methylisothiopseudouronium
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Topics |
- Animals
- Burns
(complications, epidemiology)
- Comorbidity
- Enzyme Inhibitors
(therapeutic use)
- Interleukin-1beta
(metabolism)
- Isothiuronium
(analogs & derivatives, therapeutic use)
- Lipopolysaccharides
(adverse effects)
- Lung Injury
(drug therapy, etiology, metabolism)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Models, Animal
- Nitric Oxide Synthase Type II
(antagonists & inhibitors, metabolism)
- Pseudomonas Infections
(complications, epidemiology)
- Pseudomonas aeruginosa
- Receptors, Tumor Necrosis Factor, Type I
(deficiency, genetics, metabolism)
- Toll-Like Receptor 4
(deficiency, genetics, metabolism)
- Treatment Outcome
- Tumor Necrosis Factor-alpha
(therapeutic use)
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