Abstract | OBJECTIVE: METHODS: The proteins in the brain tissue were extracted by two-dimensional gel electrophoresis in 3 children undergoing brain ventricular neoplasms resection (normal brain tissue) and in 8 children with contusion and laceration of brain. The image analysis was done using the PDQuest 7.0 software. The differential protein spots were detected and analyzed with Applied Biosystems Voyager System 4307 MALDI-TOF Mass Spectrometer and bioinformatical skills. Ngb expression in the brain tissue was measured using immunohistochemisty. Ngb expression in plasma was measured using ELISA in 15 children with contusion and laceration of brain and 10 healthy children. RESULTS: Expression maps of the brain tissue were established by two-dimensional gel electrophoresis in children with contusion and laceration of brain and healthy children. Six differential protein spots were found and 5 of them were identified by mass spectrum. Immunohistochemisty assay showed that Ngb expression in the brain tissue in children with contusion and laceration of brain was significantly higher than in normal controls (P<0.05). ELISA results showed that Ngb expression in the plasma increased significantly 6, 12, 18, 24 and 48 hours after trauma in children with contusion and laceration of brain compared with healthy children (P<0.01). CONCLUSIONS: Ngb may play an important role in the pathologic process of contusion and laceration of brain in children.
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Authors | Zhen-Yan Li, Zheng-Hao Deng, Chun-Tao Li, Yong-Jun Tang, Guang-Wei Zhong, Wei Li, Yun-Sheng Liu, Jing-Ping Liu |
Journal | Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
(Zhongguo Dang Dai Er Ke Za Zhi)
Vol. 14
Issue 9
Pg. 697-702
(Sep 2012)
ISSN: 1008-8830 [Print] China |
PMID | 22989442
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Nerve Tissue Proteins
- Neuroglobin
- Globins
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Topics |
- Adolescent
- Brain Injuries
(metabolism)
- Child
- Electrophoresis, Gel, Two-Dimensional
- Enzyme-Linked Immunosorbent Assay
- Female
- Globins
(analysis)
- Humans
- Immunohistochemistry
- Male
- Nerve Tissue Proteins
(analysis)
- Neuroglobin
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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