Cytokine factors present in dengue patient sera induces alterations of junctional proteins in human endothelial cells.

Abstract

Plasma leakage in severe dengue has been postulated to be associated with skewed cytokine immune responses. In this study, the association of cytokines with vascular permeability in dengue patients was investigated. Human serum samples collected from 48 persons (13 with dengue fever, 29 with dengue hemorrhagic fever, and 6 healthy) were subjected to cytokines analysis by using Luminex Multiplex Technology. Selected serum samples from patients with dengue hemorrhagic fever sera and recombinant human cytokines were then tested for roles on inducing vascular permeability by treatment of human umbilical vein endothelial cells. Confocal immunofluorescence staining indicated morphologic alteration of human umbilical vein endothelial cells treated with serum samples from patients with dengue hemorrhagic fever compared with serum samples from healthy persons. The findings suggest that cytokines produced during dengue hemorrhagic infections could induce alterations in the vascular endothelium, which may play a fundamental role in the pathophysiology of dengue.

• Authors: Ramapraba Appanna, Seok Mui Wang, Sasheela A Ponnampalavanar, Lucy Chai See Lum, Shamala Devi Sekaran
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 87 Issue 5 Pg. 936-42 (Nov 2012) ISSN: 1476-1645 [Electronic] United States
• PMID: 22987650 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines

Topics

• Cells, Cultured
• Cytokines (blood)
• Dengue (blood)
• Endothelial Cells (metabolism)
• Endothelium, Vascular (metabolism)
• Fluorescent Antibody Technique
• Humans