Abstract | AIM: METHODS: Primary astrocyte cultures subjected to oxygen- glucose deprivation (OGD) were used as an in vitro simulated ischemic model. Poly I:C was administrated 2 h before OGD. Cell toxicity was measured using MTT assay and LDH leakage assay. The levels of TNFα, IL-6 and interferon-β (IFNβ) in the media were measured using ELISA. Toll/ interleukin receptor domain-containing adaptor-inducing IFNβ (TRIF) protein levels were detected using Western blot analysis. A mouse middle cerebral artery occlusion (MCAO) model was u sed for in vivo study. The animals were administered Poly I:C (0.3 mg/kg, im) 2 h before MCAO, and examined with neurological deficit scoring and TTC staining. The levels of TNFα and IL-6 in ischemic brain were measured using ELISA. RESULTS: Pretreatment with Poly I:C (10 and 20 μg/mL) markedly attenuated OGD-induced astrocyte injury, and significantly raised the cell viability and reduced the LDH leakage. Poly I:C significantly upregulated TRIF expression accompanied by increased downstream IFNβ production. Moreover, Poly I:C significantly suppressed the pro-inflammatory cytokines TNFα and IL-6 production. In mice subjected to MCAO, administration of Poly I:C significantly attenuated the neurological deficits, reduced infarction volume, and suppressed the increased levels of TNFα and IL-6 in the ischemic striatum and cortex. CONCLUSION:
Poly I:C pretreatment exerts neuroprotective and anti-inflammatory effects in the simulated cerebral ischemia models, and the neuroprotection is at least in part due to the activation of the TLR3-TRIF pathway.
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Authors | Lin-na Pan, Wei Zhu, Cai Li, Xu-lin Xu, Lian-jun Guo, Qing Lu |
Journal | Acta pharmacologica Sinica
(Acta Pharmacol Sin)
Vol. 33
Issue 10
Pg. 1246-53
(Oct 2012)
ISSN: 1745-7254 [Electronic] United States |
PMID | 22983393
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Culture Media
- Neuroprotective Agents
- Toll-Like Receptor 3
- Glucose
- Poly I-C
- Oxygen
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Topics |
- Animals
- Astrocytes
(drug effects, metabolism)
- Blotting, Western
- Cell Culture Techniques
- Cell Survival
(drug effects)
- Cells, Cultured
- Culture Media
- Disease Models, Animal
- Enzyme-Linked Immunosorbent Assay
- Glucose
(metabolism)
- Ischemic Attack, Transient
(metabolism, prevention & control)
- Mice
- Neuroprotective Agents
(pharmacology, therapeutic use)
- Oxygen
(metabolism)
- Poly I-C
(pharmacology, therapeutic use)
- Rats
- Rats, Sprague-Dawley
- Toll-Like Receptor 3
(agonists)
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