Potential cross-reactivity of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid (N)-based IgG ELISA assay for plasma samples from HIV-1 positive intravenous drug users (IDUs).

Abstract

AIM:

to evaluate the specificity of the SARS-CoV N protein-based IgG ELISA assay for detection of immunoglobulin G (IgG) in plasma samples obtained from HIV-1 positive and HIV-1 negative intravenous drug users (IDUs).

METHODS:

the SARS-CoV N gene was cloned into pQE-80L vector, and the constructs were transformed into Escherichia coli BL21. The 6x His-tagged N protein was expressed by inducing the bacterial cells with isopropyl-1-thio-D-galactopyranoside (IPTG) and purified by Ni-NTA affinity resin. The 6x His-tagged N protein was used as antigen for ELISA assay and evaluated for the serum samples from patients with SARS positive and the plasma samples from the HIV-1 positive and negative IDUs.

RESULTS:

all sera samples from patients with SARS positive were the ELISA positive (100% sensitivity). The ELISA assay yielded no positive results of the total 61 HIV-1 negative IDU samples (100% specificity) and two positive results of the total 68 HIV-1 positive IDU samples (97.06% specificity).

CONCLUSION:

the specificity of the SARS-CoV N protein-based IgG ELISA assay for the detection of the SARS-CoV N specific IgG in plasma samples from IDUs with HIV-1 positive is, therefore, questionable.

• Authors: Andi Yasmon, Fera Ibrahim, Budiman Bela, Agus Sjahrurachman
• Journal: Acta medica Indonesiana (Acta Med Indones) Vol. 44 Issue 3 Pg. 193-8 (Jul 2012) ISSN: 0125-9326 [Print] Indonesia
• PMID: 22983073 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Coronavirus Nucleocapsid Proteins
• Immunoglobulin G
• Nucleocapsid Proteins

Topics

• Adult
• Coronavirus Nucleocapsid Proteins
• Cross Reactions
• Enzyme-Linked Immunosorbent Assay
• HIV Seronegativity
• HIV Seropositivity (immunology, virology)
• HIV-1 (immunology)
• Humans
• Immunoglobulin G (immunology)
• Nucleocapsid Proteins (immunology)
• Severe acute respiratory syndrome-related coronavirus (immunology)
• Sensitivity and Specificity
• Serologic Tests
• Severe Acute Respiratory Syndrome (diagnosis, virology)
• Substance Abuse, Intravenous (blood)
• Young Adult