Hepatoprotective and antiviral properties of isochlorogenic acid A from Laggera alata against hepatitis B virus infection.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

The aim of this study was to determine the anti-hepatitis B effect of isochlorogenic acid A isolated from Laggera alata (Asteraceae), a traditional Chinese herbal medicine.

MATERIALS AND METHODS:

The anti-hepatitis B activity of isochlorogenic acid A was evaluated by the D-galactosamine (D-GalN)-induced HL-7702 hepatocyte damage model and the HBV-transfected HepG2.2.15 cells.

RESULTS:

Isochlorogenic acid A significantly improved HL-7702 hepatocyte viability and markedly inhibited the productions of HBsAg and HBeAg. The inhibitory rates of isochlorogenic acid A on the HBsAg and HBeAg expressions were 86.9% and 72.9%, respectively. In addition, isochlorogenic acid A declined markedly the content of hepatitis B virus covalently closed circular DNA (HBV cccDNA) and induced significantly the heme oxygenase-1 (HO-1) expression in HepG2.2.15 cells.

CONCLUSIONS:

Isochlorogenic acid A was verified to possess the potent anti-hepatitis B activity. The anti-HBV target of isochlorogenic acid A is probably associated with blocking the translation step of the HBV replication. Overexpression of HO-1 may contribute to the anti-HBV activity of isochlorogenic acid A by reducing the stability of the HBV core protein and thus blocking the refill of nuclear HBV cccDNA. Additionally, the hepatoprotective effect of isochlorogenic acid A could be achieved by its antioxidative property and induction of HO-1.

• Authors: Bing-Jie Hao, Yi-Hang Wu, Jian-Guo Wang, Shao-Qing Hu, Dana Jasmin Keil, Hua-Jun Hu, Ji-Dong Lou, Yu Zhao
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 144 Issue 1 Pg. 190-4 (Oct 31 2012) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 22982394 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Antigens, Viral
• Antiviral Agents
• DNA, Viral
• Protective Agents
• isochlorogenic acid
• Chlorogenic Acid
• HMOX1 protein, human
• Heme Oxygenase-1

Topics

• Antigens, Viral (analysis)
• Antiviral Agents (pharmacology)
• Asteraceae
• Cell Survival (drug effects)
• Chlorogenic Acid (analogs & derivatives, pharmacology)
• DNA, Viral (analysis)
• Heme Oxygenase-1 (metabolism)
• Hep G2 Cells
• Hepatitis B (prevention & control)
• Hepatitis B virus (drug effects, genetics)
• Humans
• Protective Agents (pharmacology)