A split mouth, double-blinded, randomized controlled clinical trial was conducted on 20 subjects diagnosed with
chronic periodontitis. Quadrants of each subject were randomly assigned to four groups and treated accordingly: Group A, scaling and
root planing (SRP): Group B, topical ozonated
olive oil (OZO) as an adjunct to scaling and
root planing: Group C, topical ozonated
olive oil as a monotherapy and: Group D, topical
chlorhexidine gel as a monotherapy. The quadrants were analyzed clinically by plaque index, gingival index, sulcus
bleeding index, probing pocket depth, and clinical attachment level at baseline, 2, 4, 6 and 8 weeks of time intervals. The subjects were also analyzed for perceived
pain, discomfort or tooth
hypersensitivity (quadrant wise) on a Visual Analogue Scale (VAS). Additionally, subgingival plaque samples were collected from the two predetermined sites of each quadrant at baseline, 4 and 8 weeks for the analysis of total bacterial counts (TBCs) and the detection of frequency of eight putative periodontopathogens by polymerase chain reaction (PCR) method.
RESULTS: The adjunctive use of the OZO with SRP resulted in a significant improvement (P<0.001) of clinical parameters as well as microbiological parameters over the time and in comparison to the control groups. The OZO as monotherapy also showed a significant improvement (P<0.001) in clinical parameters as well as microbiological parameters over the time without any documented side effects. However, there was a significant increase (P<0.05) in dentinal
hypersensitivity following OZO as an adjunct to scaling and
root planing therapy.
CONCLUSION: