Labeling of polyethylenimine with fluorescent dye to image nucleus, nucleolus, and chromosomes in digitonin-permeabilized HeLa cells.

Abstract

The visualization of nuclear architecture, which changes dynamically depending on the physiological and the pathological situation, remains an important challenge. Here we report that exposure of fluoresceinisothiocyanate-labeled polyethylenimine (FITC-PEI) to digitonin-permeabilized cervical cancer HeLa cells enable rapid detection of the morphology of the nuclear rim, the nucleolus, and mitotic chromosomes. This simple detection strategy can aid in scientific investigation for both basic research and diagnostic purposes.

• Authors: Masayuki Saito, Hisato Saitoh
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 76 Issue 9 Pg. 1777-80 ( 2012) ISSN: 1347-6947 [Electronic] England
• PMID: 22972325 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fluorescent Dyes
• fluorescein isothiocyanate-polyethylenimine
• Polyethyleneimine
• Fluorescein-5-isothiocyanate
• Digitonin

Topics

• Cell Membrane Permeability (drug effects)
• Cell Nucleolus (metabolism, ultrastructure)
• Chromosomes, Human (metabolism, ultrastructure)
• Digitonin (pharmacology)
• Fluorescein-5-isothiocyanate (analogs & derivatives, chemical synthesis, chemistry, metabolism)
• Fluorescent Dyes (chemistry)
• HeLa Cells
• Humans
• Mitosis (genetics)
• Molecular Imaging (methods)
• Polyethyleneimine (chemical synthesis, chemistry, metabolism)
• Staining and Labeling (methods)