Development of a quantum-dot-labelled magnetic immunoassay method for circulating colorectal cancer cell detection.

Abstract

AIM:

To detect of colorectal cancer (CRC) circulating tumour cells (CTCs) surface antigens, we present an assay incorporating cadmium selenide quantum dots (QDs) in these paper.

METHODS:

The principle of the assay is the immunomagnetic separation of CTCs from body fluids in conjunction with QDs, using specific antibody biomarkers: epithelial cell adhesion molecule antibody, and monoclonal cytokeratin 19 antibody. The detection signal was acquired from the fluorescence signal of QDs. For the evaluation of the performance, the method under study was used to isolate the human colon adenocarcinoma cell line (DLD-1) and CTCs from CRC patients' peripheral blood.

RESULTS:

The minimum detection limit of the assay was defined to 10 DLD-1 CRC cells/mL as fluorescence was measured with a spectrofluorometer. Fluorescence-activated cell sorting analysis and Real Time RT-PCR, they both have also been used to evaluate the performance of the described method. In conclusion, we developed a simple, sensitive, efficient and of lower cost (than the existing ones) method for the detection of CRC CTCs in human samples. We have accomplished these results by using magnetic bead isolation and subsequent QD fluorescence detection.

CONCLUSION:

The method described here can be easily adjusted for any other protein target of either the CTC or the host.

• Authors: Maria Gazouli, Anna Lyberopoulou, Pericles Pericleous, Spyros Rizos, Gerassimos Aravantinos, Nikolaos Nikiteas, Nicholas P Anagnou, Efstathios P Efstathopoulos
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 18 Issue 32 Pg. 4419-26 (Aug 28 2012) ISSN: 2219-2840 [Electronic] United States
• PMID: 22969208 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• Antigens, Neoplasm
• Cell Adhesion Molecules
• Epithelial Cell Adhesion Molecule
• Keratin-19

Topics

• Adenocarcinoma (diagnosis, immunology, pathology)
• Antibodies (blood)
• Antigens, Neoplasm (immunology)
• Case-Control Studies
• Cell Adhesion Molecules (immunology)
• Colorectal Neoplasms (diagnosis, immunology, pathology)
• Epithelial Cell Adhesion Molecule
• Humans
• Immunoassay (methods)
• Immunomagnetic Separation (methods)
• Keratin-19 (immunology)
• Quantum Dots
• Sensitivity and Specificity
• Spectrometry, Fluorescence