Abstract | RATIONALE AND OBJECTIVES:
Fibrin deposition has been indicated within the stroma of a majority of solid tumors. Here we assess the feasibility of using the established fibrin-specific probe EP-2104R for noninvasive imaging of fibrin in the context of breast cancer. METHODS: RESULTS:
EP-2104R transiently enhanced both tumor core and tumor periphery, but only the enhancement in the tumor periphery persisted even 90 minutes after EP-2104R administration. However, untargeted Gd-DTPA and CLP are not retained in the tumor periphery. The half-life of EP-2104R in the tumor periphery (103 ± 18 minutes) is significantly longer (P < 0.05) than that of either Gd-DTPA (29.6 ± 2.4 minutes) or CLP (42.4 ± 1.5 minutes), but the rate of clearance is similar for all the 3 probes from the tumor core. The presence of high concentrations of fibrin in the tumor periphery was corroborated using immunohistochemistry with a fibrin-specific antibody. CONCLUSIONS: The persistent enhancement observed in the tumor periphery with EP-2104R is likely a result of its fibrin-specific binding rather than its size and demonstrates the feasibility of EP-2104R for molecular imaging of fibrin in tumor stroma.
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Authors | Ritika Uppal, Zdravka Medarova, Christian T Farrar, Guangping Dai, Anna Moore, Peter Caravan |
Journal | Investigative radiology
(Invest Radiol)
Vol. 47
Issue 10
Pg. 553-8
(Oct 2012)
ISSN: 1536-0210 [Electronic] United States |
PMID | 22960948
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- EP-2104R
- Peptides
- Fibrin
- Gadolinium
- Gadolinium DTPA
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Topics |
- Adenocarcinoma
(diagnosis, pathology)
- Animals
- Breast Neoplasms
(diagnosis, pathology)
- Disease Models, Animal
- Feasibility Studies
- Female
- Fibrin
- Gadolinium
- Gadolinium DTPA
- Mice
- Molecular Imaging
- Peptides
- Transplantation, Heterologous
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