A novel method for the large-scale production of PG-CNP37, a C-type natriuretic peptide analogue.

Abstract

Achondroplasia is the most common form of human dwarfism caused by a mutation in the fibroblast growth factor receptor 3 (FGFR3), resulting in abnormal endochondral bone formation. C-type natriuretic peptide (CNP) is a potent stimulator of endochondral bone growth and represents a potential therapy for achondroplasia. We have developed a novel, simple and cost effective method to produce a CNP analogue, PG-CNP37, at a large scale from Escherichia coli. A PG-CNP37 fusion protein was over-expressed as inclusion bodies in E. coli, which were purified then cleaved by formic acid to release the PG-CNP37 peptide. Approximately 0.5g of 95% pure, soluble and active PG-CNP37 peptide was produced from 1L of culture using this method and may represent a viable means for large-scale production of other therapeutic peptides.

• Authors: Shinong Long, Daniel J Wendt, Sean M Bell, Timothy W Taylor, Jean-Yves Dewavrin, Michel C Vellard
• Journal: Journal of biotechnology (J Biotechnol) Vol. 164 Issue 2 Pg. 196-201 (Dec 15 2012) ISSN: 1873-4863 [Electronic] Netherlands
• PMID: 22959861 (Publication Type: Journal Article)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Formates
• Recombinant Fusion Proteins
• formic acid
• Natriuretic Peptide, C-Type

Topics

• Chromatography, Ion Exchange
• Cloning, Molecular
• Escherichia coli (metabolism)
• Formates
• Inclusion Bodies (metabolism)
• Natriuretic Peptide, C-Type (analogs & derivatives, chemistry, isolation & purification, metabolism)
• Recombinant Fusion Proteins (chemistry, isolation & purification, metabolism)