Abstract | PURPOSE: METHODS: To examine the effect of ATF6 on rhodopsin, wild-type (WT) or mutant rhodopsins were expressed in cells expressing inducible human ATF6f, the transcriptional activator domain of ATF6. Induction of ATF6f synthesis rapidly activated downstream genes. To examine PERK's effect on rhodopsin, WT or mutant rhodopsins were expressed in cells expressing a genetically altered PERK protein, Fv2E-PERK. Addition of the dimerizing molecule ( AP20187) rapidly activated Fv2E-PERK and downstream genes. By use of these strategies, it was examined how selective ATF6 or PERK signaling affected the fate of WT and mutant rhodopsins. RESULTS: ATF6 significantly reduced T17M, P23H, Y178C, C185R, D190G, K296E, and S334ter rhodopsin protein levels in the cells with minimal effects on monomeric WT rhodopsin protein levels. By contrast, the PERK pathway reduced both levels of WT, mutant rhodopsins, and many other proteins in the cell. CONCLUSIONS: This study indicates that selectively activating ATF6 or PERK prevents mutant rhodopsin from accumulating in cells. ATF6 signaling may be especially useful in treating retinal degenerative diseases arising from rhodopsin misfolding by preferentially clearing mutant rhodopsin and abnormal rhodopsin aggregates.
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Authors | Wei-Chieh Chiang, Nobuhiko Hiramatsu, Carissa Messah, Heike Kroeger, Jonathan H Lin |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 53
Issue 11
Pg. 7159-66
(Oct 01 2012)
ISSN: 1552-5783 [Electronic] United States |
PMID | 22956602
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- ATF6 protein, human
- Activating Transcription Factor 6
- Membrane Proteins
- Rhodopsin
- ERN2 protein, human
- PERK kinase
- Protein Serine-Threonine Kinases
- eIF-2 Kinase
- Endoribonucleases
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Topics |
- Activating Transcription Factor 6
(metabolism)
- Cell Membrane
(metabolism)
- Endoplasmic Reticulum Stress
(physiology)
- Endoribonucleases
(metabolism)
- HEK293 Cells
- Homeostasis
(physiology)
- Humans
- Membrane Proteins
(metabolism)
- Mutagenesis
(physiology)
- Protein Serine-Threonine Kinases
(metabolism)
- Proteostasis Deficiencies
(genetics, metabolism, pathology)
- Retina
(metabolism, pathology)
- Retinitis Pigmentosa
(genetics, metabolism, pathology)
- Rhodopsin
(genetics, metabolism)
- Signal Transduction
(physiology)
- Unfolded Protein Response
(physiology)
- eIF-2 Kinase
(metabolism)
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