Abstract | BACKGROUND: METHODS: AR42J acinar cells were pretreated with TNF-α to induce pancreatitis followed by 25 and 50 μM pterostilbene for 15 and 30 min. Secretion of lipase was quantified using a lipase assay and used as a marker of TNF-α-induced pancreatitis. Detection of STAT3, IL-1β, and IL-6 was performed using enzyme-linked immunosorbent assay. Analysis of variance and Tukey post hoc analysis were used for statistical analysis. RESULTS: TNF-α increased the secretion of lipase, IL-1β, and IL-6. Pterostilbene treatment inhibited TNF-α-induced secretion of lipase (P<0.01 and P<0.001), IL-1β (P<0.05), and IL-6 (P<0.05 and P<0.01). Inhibition of STAT3 by pterostilbene occurred with treatment doses of 25 and 50 μM (P<0.001 and P<0.01). CONCLUSIONS:
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Authors | Denise McCormack, Debbie McDonald, David McFadden |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 178
Issue 1
Pg. 28-32
(Nov 2012)
ISSN: 1095-8673 [Electronic] United States |
PMID | 22954523
(Publication Type: Journal Article)
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Copyright | Copyright © 2012 Elsevier Inc. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Interleukin-1beta
- Interleukin-6
- STAT3 Transcription Factor
- Stat3 protein, rat
- Stilbenes
- Tumor Necrosis Factor-alpha
- pterostilbene
- Lipase
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Topics |
- Acinar Cells
(cytology, drug effects, metabolism)
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- Cell Line
- Drug Interactions
- In Vitro Techniques
- Interleukin-1beta
(metabolism)
- Interleukin-6
(metabolism)
- Lipase
(metabolism)
- Pancreatitis
(chemically induced, drug therapy, pathology)
- Pterocarpus
(chemistry)
- Rats
- STAT3 Transcription Factor
(metabolism)
- Stilbenes
(pharmacology)
- Tumor Necrosis Factor-alpha
(pharmacology)
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