Effects of endokinin A/B and endokinin C/D on the antinociception properties of hemopressin in mice.

Abstract

The current study evaluated the effects of hemopressin (HP) on pain modulation by endokinin A/B (EKA/B) and endokinin C/D (EKC/D) at the supraspinal level in mice. Intracerebroventricular administration of HP (10 nmol) fully antagonized the hyperalgesia induced by EKA/B (10, 30, and 100 pmol), and induced a dose-dependent potent analgesic effect. HP at different concentrations (10 pmol, 100 pmol, and 1 nmol) showed varying effects on the analgesic effect of EKA/B (3 nmol). HP extended the duration of the analgesic effect of EKC/D (3 nmol). Moreover, HP at different concentrations (10 pmol, 5 pmol, 1 pmol, and 100 fmol) co-administered with EKC/D (30 pmol) induced significant analgesia at two different time points: 5 min and 50 min. To investigate the antinociceptive mechanism, we used SR140333B and SR142801. HP (1 pmol) potentiated the analgesic effect of SR140333B (100 pmol)+EKA/B (30 pmol) in 5-10 min, while HP (100 pmol) had no effect in the analgesia induced by SR140333B (3 nmol)+EKA/B (3 nmol). HP (1 nmol) fully inhibited the analgesic effect of SR140333B (3 nmol)+EKC/D (3 nmol) or SR142801 (3 nmol)+EKC/D (3 nmol). HP (1 pmol) weakened the analgesic effect of SR142801 (100 pmol)+EKA/B (30 pmol), but HP (100pmol) strengthened the analgesic effect of SR142801 (3 nmol)+EKA/B (3 nmol). These findings may pave the way for a new strategy on investigating the interaction between tachykinins and opioids on pain modulation.

• Authors: Lanxia Zhou, Qiaoying Jin, Yinliang Yang, Zhengkun Liu, Xiaoli Li, Shouliang Dong, Li Zhao
• Journal: Peptides (Peptides) Vol. 38 Issue 1 Pg. 70-80 (Nov 2012) ISSN: 1873-5169 [Electronic] United States
• PMID: 22951322 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• Analgesics
• Hemoglobins
• Neurokinin-1 Receptor Antagonists
• Peptide Fragments
• Piperidines
• Receptors, Neurokinin-3
• SR140333B
• Tachykinins
• Tropanes
• endokinin A-B, human
• endokinin C-D, human
• hemopressin
• SR 142801

Topics

• Analgesics (pharmacology)
• Animals
• Dose-Response Relationship, Drug
• Hemoglobins (administration & dosage, pharmacology)
• Hyperalgesia (chemically induced, drug therapy)
• Injections, Intraventricular
• Male
• Mice
• Neurokinin-1 Receptor Antagonists
• Peptide Fragments (administration & dosage, pharmacology)
• Piperidines (pharmacology)
• Receptors, Neurokinin-3 (antagonists & inhibitors)
• Tachykinins (pharmacology)
• Tropanes (pharmacology)