Abstract | BACKGROUND: METHODS: A-07-GFP and R-18-GFP human melanoma xenografts grown in dorsal window chambers were used as preclinical tumor models. Morphologic parameters of tumor vascular networks were assessed from high-resolution transillumination images, and tumor blood supply time was assessed from first-pass imaging movies recorded after a bolus of 155 kDa tetramethylrhodamine isothiocyanate-labeled dextran had been administered intravenously. Tumor hypoxia was assessed from immunohistochemical preparations of the imaged tissue by use of pimonidazole as a hypoxia marker. RESULTS:
Sunitinib treatment reduced vessel densities, increased vessel segment lengths, did not affect blood supply times, and increased hypoxic area fractions. CONCLUSION:
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Authors | Jon-Vidar Gaustad, Trude G Simonsen, Marit N Leinaas, Einar K Rofstad |
Journal | BMC cancer
(BMC Cancer)
Vol. 12
Pg. 388
(Sep 04 2012)
ISSN: 1471-2407 [Electronic] England |
PMID | 22947392
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Angiogenesis Inhibitors
- Antineoplastic Agents
- Indoles
- Pyrroles
- Sunitinib
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Topics |
- Angiogenesis Inhibitors
(administration & dosage, pharmacology)
- Animals
- Antineoplastic Agents
(administration & dosage, pharmacology)
- Basement Membrane
(drug effects)
- Blood Vessels
(drug effects, metabolism, pathology)
- Cell Hypoxia
- Female
- Humans
- Indoles
(administration & dosage, pharmacology)
- Melanoma
(blood supply, drug therapy, pathology)
- Mice
- Mice, Nude
- Neovascularization, Pathologic
(drug therapy, pathology)
- Pyrroles
(administration & dosage, pharmacology)
- Spheroids, Cellular
- Sunitinib
- Tumor Burden
(drug effects)
- Tumor Cells, Cultured
- Xenograft Model Antitumor Assays
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