[Induction effects of pentachlorophenol on vitellogenin and p53 in Chinese rare minnow (Gobiocypris rarus)].

Taking the Chinese rare minnow (Gobiocypris rarus) as the test animal, the studies were designed to investigate induction effects of pentachlorophenol (PCP) on vitellogenin (VTG) protein, VTG gene and tumor suppressor p53 gene in the liver of Gobiocypris rarus. The endocrine disrupting of PCP was evaluated by detecting VTG, and sensitive biomarkers of PCP were screened at both protein and mRNA levels. Gobiocypris rarus were exposed to PCP at 1.5, 15, 40, 80, 120, 150, 160 microg x L(-1) respectively, while setting blank, solvent control and 17alpha-ethynylestradiol (EE2) as positive control. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), enzyme-linked immunosorbent assay (ELISA), VTG protein expression differences were detected in the liver of Gobiocypris rarus after exposure to PCP. Cloning the VTG and p53 gene new fragments of Gobiocypris rarus based on conserved regions, mRNA expression levels of VTG and p53 gene in the liver of Gobiocypris rarus were determined by quantitative real-time PCR assay after PCP treatment. The results showed that 40, 80, 120, 160 microg x L(-1) PCP induced the liver of male and female Gobiocypris rarus to produce VTG protein, and had a significant concentration effect. VTG and p53 mRNA levels significantly increased in the liver of Gobiocypris rarus after exposure to 1.5, 15, 150 microg x L(-1) PCP, and had remarkable concentration and time effects. The studies suggested that PCP had estrogenic effects, and VTG protein, VTG and p53 gene in the liver of Gobiocypris rarus could be used as candidate sensitive biomarkers for detecting PCP.
AuthorsLi Xiong, Yong-Peng Ma, Xiao-Zheng Zhang, Bang-Ming Jin, Wei Li, Yong-Liang Su, Si-Yu Mao, Yan Liu
JournalHuan jing ke xue= Huanjing kexue / [bian ji, Zhongguo ke xue yuan huan jing ke xue wei yuan hui "Huan jing ke xue" bian ji wei yuan hui.] (Huan Jing Ke Xue) Vol. 33 Issue 6 Pg. 1858-64 (Jun 2012) ISSN: 0250-3301 [Print] China
PMID22946166 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Endocrine Disruptors
  • Estrogens
  • RNA, Messenger
  • Tumor Suppressor Protein p53
  • Vitellogenins
  • Water Pollutants, Chemical
  • Pentachlorophenol
  • Animals
  • Cyprinidae (metabolism)
  • Endocrine Disruptors (toxicity)
  • Environmental Monitoring (methods)
  • Estrogens
  • Liver (drug effects, metabolism)
  • Pentachlorophenol (toxicity)
  • RNA, Messenger (genetics, metabolism)
  • Tumor Suppressor Protein p53 (genetics, metabolism)
  • Vitellogenins (genetics, metabolism)
  • Water Pollutants, Chemical (toxicity)

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