Mechanism and efficacy of mobilization of granulocyte colony-stimulating factor in the treatment of chronic hepatic failure.

Abstract

BACKGROUND/AIMS:

To explore the efficacy of G-CSF mobilization in the treatment of chronic liver failure (CLF) and the mechanism of its action.

METHODOLOGY:

The proportions of cluster-of-differentiation (CD)-34+ cells and their receptor-CXCR4 were detected by flow cytometry in patients with different types of chronic HBV infection. The levels of chemokines and cytokines were measured by enzyme-linked immunosorbent assay.

RESULTS:

The proportion of CD34+ cells in patients with cirrhosis was significantly increased compared with the healthy controls (p<0.05) and was increased obviously after treatment by G-CSF mobilization (p<0.01). The expression levels of SDF-1, SCF and MMP-9 were significantly elevated in patients with chronic hepatitis B and liver cirrhosis (p<0.01). The expression levels of SCF and MMP-9 were significantly elevated after treatment with G-CSF (p<0.05). No significant differences were found in the levels of total bilirubin, albumin and prothrombin time between the treated and control groups; furthermore, no significant differences were observed in the cure and improvement rates between the two groups.

CONCLUSIONS:

The basal levels of stem cell mobilization in patients with liver cirrhosis might be associated with the repair of liver injury. G-CSF could promote hematopoietic stem cell mobilization through regulation of the expression levels of stem-cell-mobilization-related factors in patients with liver cirrhosis. No apparent effects of G-CSF therapy on both liver function and short-term prognosis in patients with liver cirrhosis were confirmed.

• Authors: Tong-Jing Xing, Hong-Tao Xu, Jian-Chun Xian, Mei-Long Shen, Hao Li, Jun Ye, Li-Xin Zhang
• Journal: Hepato-gastroenterology (Hepatogastroenterology) 2013 Jan-Feb Vol. 60 Issue 121 Pg. 170-5 ISSN: 0172-6390 [Print] Greece
• PMID: 22945339 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CXCL12 protein, human
• CXCR4 protein, human
• Chemokine CXCL12
• Receptors, CXCR4
• Stem Cell Factor
• Granulocyte Colony-Stimulating Factor
• Matrix Metalloproteinase 9

Topics

• Adult
• Chemokine CXCL12 (analysis)
• End Stage Liver Disease (drug therapy, immunology)
• Female
• Flow Cytometry
• Granulocyte Colony-Stimulating Factor (therapeutic use)
• Hematopoietic Stem Cell Mobilization
• Humans
• Male
• Matrix Metalloproteinase 9 (analysis)
• Middle Aged
• Receptors, CXCR4 (analysis)
• Stem Cell Factor (analysis)