Biological response modifiers influence structure function relationship of hematopoietic stem and stromal cells in a mouse model of leukemia.

Abstract

Biological response modifiers (BRMs) can alter interactions between the immune system and cancer cells to boost, direct, or restore the body's ability to fight disease. Mice with ethylnitrosourea- (ENU) induced leukemia were here used to monitor the therapeutic efficacy of lipopolysaccaride (LPS), Bacillus Calmette Guerin (BCG) and sheep erythrocytes (SRBC). Flow cytometry based CD34+ positivity analysis, clonogenicity, proliferation and ultrastructure studies using scanning electron microscopy (SEM) of stem cells in ENU induced animals with and without BRMs treatment were performed. BRMs improved the stem-stromal relationship structurally and functionally and might have potential for use as an adjunct in human stem cell therapy.

• Authors: Kaustuv Basu, Joydeep Mukherjee, Sujata Law, Samaresh Chaudhuri
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 13 Issue 6 Pg. 2935-41 ( 2012) ISSN: 2476-762X [Electronic] Thailand
• PMID: 22938486 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD34
• BCG Vaccine
• Immunologic Factors
• Lipopolysaccharides
• Ethylnitrosourea

Topics

• Animals
• Antigens, CD34 (analysis)
• BCG Vaccine (immunology, pharmacology)
• Cell Proliferation
• Cells, Cultured
• Erythrocytes (immunology)
• Ethylnitrosourea
• Hematopoietic Stem Cells (cytology, drug effects, immunology, physiology, ultrastructure)
• Immunologic Factors (pharmacology)
• Leukemia, Experimental (immunology, pathology, therapy)
• Lipopolysaccharides (immunology, pharmacology)
• Mice
• Sheep
• Stromal Cells (drug effects, immunology, physiology)