The influence of 8-prenylnaringenin on the activity of voltage-gated Kv1.3 potassium channels in human Jurkat T cells.

Abstract

Using the whole-cell patch-clamp technique, we investigated the influence of 8-prenylnaringenin on the activity of the voltage-gated Kv1.3 potassium channels in the human leukemic T lymphocyte cell line Jurkat. 8-prenylnaringenin is a potent plant-derived phytoestrogen that has been found to inhibit cancer cell proliferation. The results show that it inhibited the Kv1.3 channels in a concentration-dependent manner. Complete inhibition occurred at concentrations higher than 10 μM. The inhibitory effect of 8-prenylnaringenin was reversible. It was accompanied by a significant acceleration of channel inactivation without any pronounced change in the activation rate. Of the naringenin derivatives tested to date, 8-prenylnaringenin is the most potent inhibitor of the Kv1.3 channels. The potency of the inhibition may be due to the presence of a prenyl group in the molecule of this flavonoid. The inhibition of the Kv1.3 channels might be involved in the antiproliferative and pro-apoptotic effects of 8-prenylnaringenin that have been observed in cancer cell lines expressing these channels.

• Authors: Justyna Gąsiorowska, Andrzej Teisseyre, Anna Uryga, Krystyna Michalak
• Journal: Cellular & molecular biology letters (Cell Mol Biol Lett) Vol. 17 Issue 4 Pg. 559-70 (Dec 2012) ISSN: 1689-1392 [Electronic] England
• PMID: 22933043 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 8-prenylnaringenin
• Flavanones
• Kv1.3 Potassium Channel

Topics

• Apoptosis (drug effects)
• Cell Proliferation (drug effects)
• Flavanones (pharmacology)
• Humans
• Jurkat Cells
• Kv1.3 Potassium Channel (antagonists & inhibitors, metabolism)
• Patch-Clamp Techniques