Effect of inhibitors of cytoplasmic structures and functions on rabies virus infection in vitro.

Abstract	The effect in vitro of some cytoplasmic structure and function inhibitors on the different stages of rabies virus infection was investigated. Treatment of fibroblasts (CER) and human neuroblastoma cells (IMR-32) with substances acting on low pH intracellular compartments (methylamine and monensin) prevented rabies virus genome delivery in the cytosol. An early inhibition of viral infection was also obtained in the presence of B and D cytochalasins and trifluoperazine which interact with microfilament structures. Treatment with colchicine and vinblastine did not affect rabies multiplication, suggesting that microtubules are not involved in this process. However, the multiplication of prebound virions did not take place in the presence of inhibitors of oxidative phosphorylation (sodium azide and CCCP) and of glycolysis (2-deoxy-D-glucose) indicating that rabies virus replication is largely energy-dependent in both host cells examined.
Authors	C Conti, F Superti, M Divizia, A Pana, N Orsi
Journal	Comparative immunology, microbiology and infectious diseases (Comp Immunol Microbiol Infect Dis) Vol. 13 Issue 3 Pg. 137-46 ( 1990) ISSN: 0147-9571 [Print] England
PMID	2292183 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Azides Methylamines Trifluoperazine Cytochalasin D Cytochalasin B Carbonyl Cyanide m-Chlorophenyl Hydrazone Vinblastine Monensin Sodium Azide Deoxyglucose methylamine Colchicine
Topics	Animals Azides (pharmacology) Carbonyl Cyanide m-Chlorophenyl Hydrazone (pharmacology) Cell Line Colchicine (pharmacology) Cytochalasin B (pharmacology) Cytochalasin D (pharmacology) Deoxyglucose (pharmacology) Dose-Response Relationship, Drug Fibroblasts Humans Methylamines (pharmacology) Monensin (pharmacology) Neuroblastoma Rabies virus (drug effects, physiology) Sodium Azide Trifluoperazine (pharmacology) Tumor Cells, Cultured Vinblastine (pharmacology) Virus Replication (drug effects)

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