Abstract | BACKGROUND: METHODS: We rendered donor dogs cardiac dead and left them at room temperature. All animals received ventilation for 60 minutes starting at 240 minutes after cardiac arrest. The animals were divided into 2 groups: NS ( normal saline, n = 7) group, which received aerosolized normal saline, and SF ( surfactant; n = 5), which received aerosolized surfactant. The lungs were flushed and procured, and the left lung was transplanted into recipient dogs. At 45 minutes of reperfusion, the right pulmonary artery was ligated, and the left transplanted lung function was evaluated. RESULTS: In the NS group, 2 of 7 dogs died at 75 minutes after reperfusion, whereas all 5 animals in the SF group survived for 240 minutes after reperfusion. The SF group showed significantly better dynamic compliance, oxygenation, and wet-to-dry weight ratio. Furthermore, the SF group had higher levels of high-energy phosphates in the lung tissues and lower levels of interleukin-8, tumor necrosis factor-α, and protein in the bronchoalveolar lavage fluid. Histologically, the lungs in the SF group showed fewer signs of interstitial edema and hemorrhage and significantly less neutrophilic sequestration than those of the NS group. CONCLUSIONS:
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Authors | Akihiro Ohsumi, Fengshi Chen, Jin Sakamoto, Daisuke Nakajima, Kyoko Hijiya, Hideki Motoyama, Kenji Okita, Kenta Horita, Ryutaro Kikuchi, Tetsu Yamada, Toru Bando, Hiroshi Date |
Journal | The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation
(J Heart Lung Transplant)
Vol. 31
Issue 10
Pg. 1136-42
(Oct 2012)
ISSN: 1557-3117 [Electronic] United States |
PMID | 22921405
(Publication Type: Journal Article)
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Copyright | Copyright © 2012 International Society for Heart and Lung Transplantation. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Cytokines
- Pulmonary Surfactants
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Topics |
- Administration, Inhalation
- Animals
- Bronchoalveolar Lavage Fluid
(chemistry)
- Cytokines
(analysis)
- Death
- Dogs
- Graft Survival
(physiology)
- Lung
(drug effects, physiology)
- Lung Compliance
(physiology)
- Lung Transplantation
(methods)
- Models, Animal
- Pulmonary Surfactants
(administration & dosage, pharmacology)
- Reperfusion Injury
(physiopathology, prevention & control)
- Tissue and Organ Procurement
- Warm Ischemia
(adverse effects)
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