Antiallergic asthma properties of brazilin through inhibition of TH2 responses in T cells and in a murine model of asthma.

Abstract

This study aimed to determine whether brazilin exhibits anti-inflammatory effects that inhibit T helper cell type II (T(H)2) responses and whether it suppresses allergic inflammation reactions in a murine model of asthma. We found that brazilin inhibited the mRNA and protein expression of interleukin (IL)-4 and IL-5 induced by phorbol myristate acetate (PMA) and cAMP in EL-4 T cells in a dose-dependent manner. Following the intratracheal instillation of brazilin in ovalbumin (OVA)-immunized mice, we found that brazilin-treated mice exhibited decreases in the release of IL-4, IL-5, IL-13, eotaxin-1, and tumor necrosis factor-α in bronchoalveolar lavage fluid (BALF); inhibited T(H)2 functioning via a decrease in IL-4 production; and exhibited attenuation of OVA-induced lung eosinophilia, airway hyperresponsiveness, and airway remodeling. These results suggest that brazilin exhibits anti-T(H)2 effects both in vitro and in vivo and may possess therapeutic potential for allergic diseases.

• Authors: Chen-Chen Lee, Chien-Neng Wang, Jaw-Jou Kang, Jiunn-Wang Liao, Bor-Luen Chiang, Hui-Chen Chen, Chien-Ming Hu, Chia-Der Lin, Shih-Hsuan Huang, Yu-Ting Lai
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 60 Issue 37 Pg. 9405-14 (Sep 19 2012) ISSN: 1520-5118 [Electronic] United States
• PMID: 22920671 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Asthmatic Agents
• Benzopyrans
• Chemokine CCL11
• Interleukin-13
• Interleukin-5
• Tumor Necrosis Factor-alpha
• Interleukin-4
• Ovalbumin
• brazilin

Topics

• Animals
• Anti-Asthmatic Agents (pharmacology, therapeutic use)
• Asthma (drug therapy, immunology)
• Benzopyrans (pharmacology, therapeutic use)
• Bronchoalveolar Lavage Fluid (immunology)
• Chemokine CCL11 (analysis)
• Disease Models, Animal
• Female
• Interleukin-13 (analysis)
• Interleukin-4 (analysis, antagonists & inhibitors, genetics)
• Interleukin-5 (analysis, antagonists & inhibitors, genetics)
• Mice
• Mice, Inbred BALB C
• Ovalbumin (immunology)
• Th2 Cells (drug effects, immunology)
• Tumor Necrosis Factor-alpha (analysis)