Abstract |
KIOM-79 is an herbal mixture of parched Puerariae radix, gingered Magnoliae cortex, Glycyrrhizae radix and Euphorbiae radix. In the present study, we determined the efficacy and possible mechanism of KIOM-79 on the advanced glycation end product (AGE)-modified bovine serum albumin (BSA)-induced apoptosis of cultured bovine retinal pericytes and rat retinal pericytes in Zucker diabetic fatty (ZDF) rats. Seven-week-old male ZDF rats were treated with KIOM-79 (50 mg/kg body weight) once a day orally for 13 weeks. KIOM-79 significantly inhibited pericyte apoptosis which were induced by the AGE-BSA treatment. The KIOM-79 treatment markedly suppressed the activation of nuclear factor-kappaB (NF-κB) through the inhibition of inhibitory κB kinase complex. In addition, the oral administration of KIOM-79 inhibited the changes in retinal vasculature (vascular hyperpermeability, acellular capillary). KIOM-79 strongly inhibited pericyte apoptosis, NF-κB activation and the expression of pro-apoptotic Bax and tumor necrosis factor-α. Our results suggest that KIOM-79 may exert inhibitory effects on AGE-induced pericyte apoptosis by blocking NF-κB activation, thereby ameliorating retinal microvascular dysfunction.
|
Authors | Junghyun Kim, Chan-Sik Kim, Eunjin Sohn, Yun Mi Lee, Kyuhyung Jo, Jin Sook Kim |
Journal | PloS one
(PLoS One)
Vol. 7
Issue 8
Pg. e43591
( 2012)
ISSN: 1932-6203 [Electronic] United States |
PMID | 22916281
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Glycation End Products, Advanced
- KIOM 79
- NF-kappa B
- Plant Extracts
- advanced glycation end products-bovine serum albumin
- Serum Albumin, Bovine
|
Topics |
- Animals
- Apoptosis
(drug effects)
- Blotting, Western
- Cells, Cultured
- Diabetic Retinopathy
(drug therapy, metabolism)
- Enzyme-Linked Immunosorbent Assay
- Glycation End Products, Advanced
(pharmacology)
- Male
- NF-kappa B
(metabolism)
- Pericytes
(cytology, drug effects, metabolism)
- Plant Extracts
(pharmacology, therapeutic use)
- Rats
- Serum Albumin, Bovine
(pharmacology)
|