Abstract | BACKGROUND AND AIM: METHODS AND RESULTS: A total of 150 subjects with hypercholesterolemia consumed a purified anthocyanin mixture (320 mg/d) or a placebo twice a day for 24 weeks in a randomized, double-blind trial. Anthocyanin consumption significantly decreased the levels of serum high sensitivity C-reactive protein ( hsCRP) (-21.6% vs. -2.5%, P = 0.001), soluble vascular cell adhesion molecule-1 (sVCAM-1) (-12.3% vs. 0.4%, P = 0.005) and plasma IL-1β (-12.8% vs. -1.3%, P = 0.019) compared to the placebo. We also found a significant difference in the LDL-cholesterol (-10.4% vs. 0.3%, P = 0.030) and HDL-cholesterol level changes (14.0% vs. -0.9%, P = 0.036) between the two groups. In cell culture assays in vitro, purified anthocyanin mixture, delphinidin-3-Ο-β-glucoside (Dp-3g) and cyanidin-3-Ο-β-glucoside (Cy-3g) inhibited IL-6 and IL-1β-induced CRP production (P < 0.05) in HepG2 cell line and LPS-induced VCAM-1 secretion (P < 0.05) in porcine iliac artery endothelial cell line respectively in a dose-dependent manner. In addition, the reduction of inflammatory cytokines associated with anthocyanin mixture was stronger when compared with the effects of Dp-3g and Cy-3g separately (P < 0.05). CONCLUSIONS:
Anthocyanin mixture reduced the inflammatory response in hypercholesterolemic subjects. In addition, different anthocyanin compounds were found to have additive or synergistic effects in mediating anti-inflammatory responses in vitro cell culture assays.
|
Authors | Y Zhu, W Ling, H Guo, F Song, Q Ye, T Zou, D Li, Y Zhang, G Li, Y Xiao, F Liu, Z Li, Z Shi, Y Yang |
Journal | Nutrition, metabolism, and cardiovascular diseases : NMCD
(Nutr Metab Cardiovasc Dis)
Vol. 23
Issue 9
Pg. 843-9
(Sep 2013)
ISSN: 1590-3729 [Electronic] Netherlands |
PMID | 22906565
(Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2012 Elsevier B.V. All rights reserved. |
Chemical References |
- Anthocyanins
- Anti-Inflammatory Agents
- Cholesterol, HDL
- Cholesterol, LDL
- Interleukin-1beta
- Interleukin-6
- Vascular Cell Adhesion Molecule-1
- C-Reactive Protein
|
Topics |
- Anthocyanins
(administration & dosage)
- Anti-Inflammatory Agents
(administration & dosage)
- Atherosclerosis
- C-Reactive Protein
(metabolism)
- Cholesterol, HDL
(blood)
- Cholesterol, LDL
(blood)
- Double-Blind Method
- Female
- Hep G2 Cells
- Humans
- Hypercholesterolemia
(blood, drug therapy)
- Interleukin-1beta
(blood)
- Interleukin-6
(blood)
- Male
- Middle Aged
- Vascular Cell Adhesion Molecule-1
(blood)
|