Entrapment of Sorghum root oxalate oxidase into polyvinyl alcohol membrane.

Abstract

A Cl- and NO3- insensitive oxalate oxidase, purified from the roots of 10-day old seedlings of grain Sorghum has been immobilized on polyvinyl alcohol (PVA) membrane through entrapment with 96.07% retention of initial activity. The membrane bound enzyme showed an increase in optimum pH (from 5.0 to 6.5), time of incubation (from 5 to 10 min) and Km for oxalate (from 0.38 to 6.23 mM), but decrease in incubation temperature for maximum activity (from 37 to 30 degrees C) and Vmax (from 70 nmol/min/ml to 9.7 nmol H2O2/min) and was unaffected by Cl- and NO3. The membrane bound enzyme lost 50% of its initial activity after 30 days of storage at room temperature. The use of membrane bound oxalate oxidase in determination of serum oxalate of urinary stone patients is demonstrated.

• Authors: Geeta Singh, Suman, Durgesh N Tanwar, C S Pundir
• Journal: Indian journal of biochemistry & biophysics (Indian J Biochem Biophys) Vol. 39 Issue 6 Pg. 397-400 (Dec 2002) ISSN: 0301-1208 [Print] India
• PMID: 22905397 (Publication Type: Journal Article)

Chemical References

• Oxalates
• Polyvinyl Alcohol
• Oxalic Acid
• Hydrogen Peroxide
• Oxidoreductases
• oxalate oxidase

Topics

• Cell Membrane (metabolism)
• Hydrogen Peroxide (chemistry)
• Hydrogen-Ion Concentration
• Kinetics
• Oxalates (chemistry)
• Oxalic Acid (chemistry)
• Oxidoreductases (chemistry)
• Plant Roots (metabolism)
• Polyvinyl Alcohol (chemistry)
• Sorghum (metabolism)
• Temperature
• Time Factors
• Urinary Calculi (chemistry)