Mitochondrial accumulation under oxidative stress is due to defects in autophagy.

Abstract

Mitochondrial dynamics maintains normal mitochondrial function by degrading damaged mitochondria and generating newborn mitochondria. The accumulation of damaged mitochondria influences the intracellular environment by promoting mitochondrial dysfunction, and thus initiating a vicious cycle. Oxidative stress induces mitochondrial malfunction, which is involved in many cardiovascular diseases. However, the mechanism of mitochondrial accumulation in cardiac myoblasts remains unclear. We observed mitochondrial dysfunction and an increase in mitochondrial mass under the oxidative conditions produced by tert-butyl hydroperoxide (tBHP) in cardiac myoblast H9c2 cells. However, in contrast to the increase in mitochondrial mass, mitochondrial DNA (mtDNA) decreased, suggesting that enhanced mitochondrial biogenesis may be not the primary cause of the mitochondrial accumulation. Therefore, we investigated changes in a number of proteins involved in autophagy. Beclin1, Atg12-Atg5 conjugate, Atg7 contents decreased but LC3-II accumulated in tBHP-treated H9c2 cells. Moreover, the capacity for acid hydrolysis decreased in H9c2 cells. We also demonstrated a decrease in DJ-1 protein under the oxidative conditions that deregulate mitochondrial dynamics. These results reveal that autophagy became defective under oxidative stress. We therefore suggest that defects in autophagy mediate mitochondrial accumulation under these conditions.

• Authors: Cheng Luo, Yan Li, Hui Wang, Zhihui Feng, Yuan Li, Jiangang Long, Jiankang Liu
• Journal: Journal of cellular biochemistry (J Cell Biochem) Vol. 114 Issue 1 Pg. 212-9 (Jan 2013) ISSN: 1097-4644 [Electronic] United States
• PMID: 22903604 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Wiley Periodicals, Inc.

Chemical References

• Apoptosis Regulatory Proteins
• Beclin-1
• Becn1 protein, rat
• DNA, Mitochondrial
• LC3 protein, rat
• Microtubule-Associated Proteins
• Protein Isoforms
• Small Ubiquitin-Related Modifier Proteins
• Adenosine Triphosphate
• tert-Butylhydroperoxide

Topics

• Adenosine Triphosphate (metabolism)
• Animals
• Apoptosis Regulatory Proteins (genetics, metabolism)
• Autophagy (drug effects, genetics)
• Beclin-1
• Cell Line
• DNA, Mitochondrial (biosynthesis, genetics)
• Gene Expression (drug effects)
• Microtubule-Associated Proteins (genetics, metabolism)
• Mitochondria, Heart (drug effects, metabolism)
• Mitochondrial Turnover (drug effects)
• Myoblasts, Cardiac (cytology, drug effects, metabolism)
• Oxidative Stress (drug effects)
• Protein Isoforms (genetics, metabolism)
• Rats
• Signal Transduction (drug effects)
• Small Ubiquitin-Related Modifier Proteins (genetics, metabolism)
• tert-Butylhydroperoxide (pharmacology)