Abstract | AIM: STUDY: Wistar rats were administered monocrotophos (0.8LD(50)) by oral gavage to elicit severe effects of acute poisoning and were sacrificed 2.5 h, 24 h, 7 days, 14 days and 1 month after poisoning. Acetylcholinesterse activity, mRNA and protein were assessed in cortex, striatum, hippocampus and cerebellum. RESULTS: Acute monocrotophos administration resulted in significant AChE inhibition (50-82%) in the rat brain regions 2.5 h after poisoning. AChE inhibition was associated with down regulation of synaptic AChE mRNA 24 h after poisoning in cortex and striatum. Partial recovery of AChE activity was observed 24 h after poisoning associated with increased catalytic efficiency (K(m)) of the enzyme. The recovery of AChE mRNA and protein levels to normal occurred in 7 days in cortex and cerebellum and over one month in striatum and hippocampus. CONCLUSION:
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Authors | Amajad Iqbal Kazi, Anna Oommen |
Journal | Neurotoxicology
(Neurotoxicology)
Vol. 33
Issue 5
Pg. 1284-90
(Oct 2012)
ISSN: 1872-9711 [Electronic] Netherlands |
PMID | 22903060
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Elsevier Inc. All rights reserved. |
Chemical References |
- Insecticides
- RNA, Messenger
- Monocrotophos
- Acetylcholinesterase
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Topics |
- Acetylcholinesterase
(genetics, metabolism)
- Analysis of Variance
- Animals
- Brain
(drug effects, enzymology)
- Dose-Response Relationship, Drug
- Gene Expression Regulation, Enzymologic
(drug effects)
- Insecticides
(toxicity)
- Monocrotophos
(toxicity)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Wistar
- Time Factors
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