Passive immunization with tenascin-R (TN-R) polyclonal antibody promotes axonal regeneration and functional recovery after spinal cord injury in rats.

Abstract

Tenascin-R (TN-R) is a neural specific protein and an important molecule involved in inhibition of axonal regeneration after spinal cord injury (SCI). Here we report on rabbit-derived TN-R polyclonal antibody, which acts as a TN-R antagonist with high titer and high specificity, promoted neurite outgrowth and sprouting of rat cortical neurons cultured on the inhibitory TN-R substrate in vitro. When locally administered into the lesion sites of rats received spinal cord dorsal hemisection, these TN-R antibodies could significantly decrease RhoA activation and improve functional recovery from corticospinal tract (CST) transection. Thus, passive immunotherapy with specific TN-R antagonist may represent a promising repair strategy following acute SCI.

• Authors: Jian You, Sun-Quan Hong, Mao-Ying Zhang, Hai-Lin Zhao, Tian-Zhu Liu, Hong-Long Zhou, Ying-Qian Cai, Zhi-Min Xu, Yang Guo, Xiao-Dan Jiang, Ru-Xiang Xu
• Journal: Neuroscience letters (Neurosci Lett) Vol. 525 Issue 2 Pg. 129-34 (Sep 13 2012) ISSN: 1872-7972 [Electronic] Ireland
• PMID: 22902990 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Antibodies
• Tenascin
• tenascin R
• rhoA GTP-Binding Protein

Topics

• Animals
• Animals, Newborn
• Antibodies (pharmacology, therapeutic use)
• Axons (drug effects, physiology)
• Cells, Cultured
• Female
• Hindlimb (physiopathology)
• Immunization, Passive
• Motor Activity
• Nerve Regeneration
• Neurites (drug effects, physiology)
• Rabbits
• Rats
• Rats, Sprague-Dawley
• Spinal Cord Injuries (immunology, physiopathology, therapy)
• Tenascin (antagonists & inhibitors, immunology)
• rhoA GTP-Binding Protein (metabolism)