Efficient generation, purification, and expansion of CD34(+) hematopoietic progenitor cells from nonhuman primate-induced pluripotent stem cells.

Abstract

Induced pluripotent stem cell (iPSC) therapeutics are a promising treatment for genetic and infectious diseases. To assess engraftment, risk of neoplastic formation, and therapeutic benefit in an autologous setting, testing iPSC therapeutics in an appropriate model, such as the pigtail macaque (Macaca nemestrina; Mn), is crucial. Here, we developed a chemically defined, scalable, and reproducible specification protocol with bone morphogenetic protein 4, prostaglandin-E2 (PGE2), and StemRegenin 1 (SR1) for hematopoietic differentiation of Mn iPSCs. Sequential coculture with bone morphogenetic protein 4, PGE2, and SR1 led to robust Mn iPSC hematopoietic progenitor cell formation. The combination of PGE2 and SR1 increased CD34(+)CD38(-)Thy1(+)CD45RA(-)CD49f(+) cell yield by 6-fold. CD34(+)CD38(-)Thy1(+)CD45RA(-)CD49f(+) cells isolated on the basis of CD34 expression and cultured in SR1 expanded 3-fold and maintained this long-term repopulating HSC phenotype. Purified CD34(high) cells exhibited 4-fold greater hematopoietic colony-forming potential compared with unsorted hematopoietic progenitors and had bilineage differentiation potential. On the basis of these studies, we calculated the cell yields that must be achieved at each stage to meet a threshold CD34(+) cell dose that is required for engraftment in the pigtail macaque. Our protocol will support scale-up and testing of iPSC-derived CD34(high) cell therapies in a clinically relevant nonhuman primate model.

• Authors: Jennifer L Gori, Devikha Chandrasekaran, John P Kowalski, Jennifer E Adair, Brian C Beard, Sunita L D'Souza, Hans-Peter Kiem
• Journal: Blood (Blood) Vol. 120 Issue 13 Pg. e35-44 (Sep 27 2012) ISSN: 1528-0020 [Electronic] United States
• PMID: 22898598 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD34
• Bone Morphogenetic Protein 4
• Purines
• RNA, Messenger
• StemRegenin 1
• Wnt Proteins
• Dinoprostone

Topics

• Animals
• Antigens, CD34 (metabolism)
• Blotting, Western
• Bone Morphogenetic Protein 4 (genetics, metabolism)
• Cell Differentiation
• Cell Lineage
• Cells, Cultured
• Colony-Forming Units Assay
• Dinoprostone (genetics, metabolism)
• Enzyme-Linked Immunosorbent Assay
• Flow Cytometry
• Hematopoietic Stem Cells (cytology, metabolism)
• Induced Pluripotent Stem Cells (cytology)
• Lymphocytes (cytology, metabolism)
• Macaca
• Myeloid Cells (cytology, metabolism)
• Purines (metabolism)
• RNA, Messenger (genetics)
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction
• Virus Internalization
• Wnt Proteins (genetics, metabolism)