The preparation of leukemia cell vaccine expressing BCG heat shock protein 70 and anti-leukemia effect in vitro.

Abstract

Gene-modified cell vaccines are the best way to achieve the immunotherapy for all types of acute leukemia. In this study, heat shock protein 70 (HSP70) gene of BCG was transfected into the acute leukemia cells and its anti-leukemia effect was further studied. Results showed that short-term culture of the leukemia cells exhibited increased number and no change in antigen expression. After HSP70 gene transfection, the yellow-green fluorescence on the leukemia cell surface was observed under a fluorescence microscope. The immunogenicity of HSP70-transfected cells exhibited that autologous lymphocytes proliferated significantly and secreted higher amount of IFN-γ, and cytotoxic T lymphocytes induced more beneficial anti-leukemia effects. These results suggested that gene transfection of BCG HSP70 could significantly enhance the immunogenicity of leukemia cells. It may be used as a suitable candidate gene-modified cell vaccine for cancer immunotherapy.

• Authors: Xiao-Ling Li, Li-Rong Sun, Zhi Wang, Xiu-Fang Sun
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 14 Issue 3 Pg. 235-42 (Nov 2012) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 22892217 (Publication Type: Journal Article)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Bacterial Proteins
• Cancer Vaccines
• HSP70 Heat-Shock Proteins

Topics

• Bacterial Proteins (genetics)
• Cancer Vaccines
• Cell Survival
• HSP70 Heat-Shock Proteins (genetics)
• Humans
• Immunotherapy
• Leukemia (immunology)
• Mycobacterium bovis (metabolism)
• T-Lymphocytes (immunology)
• Tumor Cells, Cultured